Effect of protein malnutrition on hematopoiesis. marrow endothelial cell involvement and action of granulocytic colony stimulating factor (G-CSF)

• Grant number: 18/25813-8
• Support Opportunities: Regular Research Grants
• Duration: June 01, 2019 - May 31, 2021
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Ricardo Ambrósio Fock
• Grantee: Ricardo Ambrósio Fock
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Primavera Borelli Garcia

Abstract

Hematopoiesis occurs in the bone marrow in regions named microenvironments, which modulate the processes of differentiation, proliferation and self-renewal of the hematopoietic stem cell (HSC). In recent years the literature has given attention to the role of medullary endothelial cells in the ability to modulate and sustain hematopoiesis associated with endothelial cells. The role of mesenchymal stem cells in the formation of these inducible microenvironments is also highlighted, either by direct contact or even by the synthesis of mediators capable of modulating hematopoietic cells. In view of the above, and based on previous data that prove that protein malnutrition compromises hematopoiesis triggering bone marrow hypoplasia with compromised hematopoietic cell production and maturation, especially of the granulocytic lineage, this project aims to uncover some regulatory aspects of hematopoiesis committed in protein malnutrition, prioritizing the study of the mechanisms involved in the participation of endothelial cells, as well as its mediation via granulocytic growth factor on the regulation of hematopoiesis. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NOGUEIRA-PEDRO, AMANDA; MAKIYAMA, EDSON NAOTO; SEGRETO, HELENA REGINA COMODO; FOCK, RICARDO AMBROSIO. The Role of Low-Dose Radiation in Association with TNF-alpha on Immunomodulatory Properties of Mesenchymal Stem Cells. STEM CELL REVIEWS AND REPORTS, v. 17, n. 3, NOV 2020. (18/25813-8)

NOGUEIRA-PEDRO, AMANDA; MAKIYAMA, EDSON NAOTO; SEGRETO, HELENA REGINA COMODO; FOCK, RICARDO AMBROSIO. The Role of Low-Dose Radiation in Association with TNF-alpha on Immunomodulatory Properties of Mesenchymal Stem Cells. STEM CELL REVIEWS AND REPORTS, v. 17, n. 3, p. 13-pg., 2020-11-18. (18/25813-8)

NEVES, BRUNA ROBERTA OLIVEIRA; DE FREITAS, SUMARA; BORELLI, PRIMAVERA; ROGERO, MARCELO MACEDO; FOCK, RICARDO AMBROSIO. Delphinidin-3-O-glucoside in vitro suppresses NF-kB and changes the secretome of mesenchymal stem cells affecting macrophage activation. NUTRITION, v. 105, p. 9-pg., 2023-01-01. (18/25813-8, 21/08658-1)

HASTREITER, ARACELI APARECIDA; DOS SANTOS, GUILHERME G.; MAKIYAMA, EDSON NAOTO; SANTOS, ED WILSON CAVALCANTE; BORELLI, PRIMAVERA; FOCK, RICARDO AMBROSIO. Effects of protein malnutrition on hematopoietic regulatory activity of bone marrow mesenchymal stem cells. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v. 93, JUL 2021. (18/25813-8, 14/06872-2)

NOGUEIRA-PEDRO, AMANDA; COMODO SEGRETO, HELENA REGINA; HELD, KATHRYN D.; GENTIL FERREIRA JUNIOR, ANTONIO FRANCISCO; DIAS, CAROLINA CARVALHO; HASTREITER, ARACELI APARECIDA; MAKIYAMA, EDSON NAOTO; PAREDES-GAMERO, EDGAR JULIAN; BORELLI, PRIMAVERA; FOCK, RICARDO AMBROSIO. Direct ionizing radiation and bystander effect in mouse mesenchymal stem cells. International Journal of Radiation Biology, v. N/A, p. 11-pg., 2022-04-22. (18/25813-8, 13/23285-0)

LIMA, FABIANA DA SILVA; FOCK, RICARDO AMBROSIO. A Review of the Action of Magnesium on Several Processes Involved in the Modulation of Hematopoiesis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 19, OCT 2020. (18/25813-8)

ANTUNES SANTOS, ANDRESSA CRISTINA; SARTORI, TALITA; BORELLI, PRIMAVERA; FOCK, RICARDO AMBROSIO. Prostaglandin F2 alpha in vitro can affect basic inflammatory parameters of mesenchymal stem cells and slight modulating some of their immunomodulatory properties. PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, v. 163, DEC 2020. (18/25813-8)

HASTREITER, ARACELI APARECIDA; DOS SANTOS, GUILHERME GALVAO; SANTOS, ED WILSON CAVALCANTE; MAKIYAMA, EDSON NAOTO; BORELLI, PRIMAVERA; FOCK, RICARDO AMBROSIO. Protein malnutrition impairs bone marrow endothelial cells affecting hematopoiesis. Clinical Nutrition, v. 39, n. 5, p. 1551-1559, MAY 2020. (18/25813-8, 14/06872-2)

APARECIDA HASTREITER, ARACELI; MAKIYAMA, EDSON NAOTO; BORELLI, PRIMAVERA; FOCK, RICARDO AMBROSIO. Impairment of G-CSF receptor on granulocytic progenitor cells causes neutropenia in protein malnutrition. NUTRITION, v. 69, JAN 2020. (18/25813-8)