| Full text | |
| Author(s): |
Catarino, Carolina Motter
[1, 2]
;
Pedrosa, Tatiana do Nascimento
[2]
;
Pennacchi, Paula Comune
[2]
;
de Assis, Silvia Romano
[2]
;
Gimenes, Fabricia
[3]
;
Lopes Consolaro, Marcia Edilaine
[3]
;
de Moraes Barros, Silvia Berlanga
[2]
;
Maria-Engler, Silvya Stuchi
[2]
Total Authors: 8
|
| Affiliation: | [1] Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Dept Chem & Biol Engineer, 110, 8th St, Troy, NY 12180 - USA
[2] Univ Sao Paulo, Sch Pharmaceut Sci, Clin & Toxicol Anal Dept, Av Prof Lineu Prestes 580, Bloco 17, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Estadual Maringa, Dept Clin Anal & Biomed, Ave Colombo 5790, BR-87020900 Maringa, Parana - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS; v. 125, p. 51-57, APR 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
Currently, there is a strong global trend towards the development of in vitro models to replace the use of animals in safety evaluation tests. Reconstructed Human Epidermis (RHE) models have been employed as an alternative method to animal testing of skin corrosion and irritation potential of chemical compounds. However, the consequences of an absence of the dermal compartment in these models should be considered since the cross-talk between fibroblasts and keratinocytes is fundamental for promoting proper epidermal stratification, homeostasis, inflammatory response and wound healing. In this study, we compare in-house developed models of Reconstructed Human Epidermis (i.e. USP-RHE) and full thickness skin (i.e. USP-FTS) regarding their response when submitted to skin corrosion assays, based on Guideline 431 (OECD). The results show that both models correctly classified the four substances tested (2-phenylethyl bromide, benzylacetone, lactic acid, octanoic acid) as corrosive or non-corrosive, Furthermore, we have demonstrated higher cell viability of the USP-FTS model compared to the USP-RHE model, a sign of its improved barrier function, following the exposure to the substances test on the corrosion assay. This emphasizes the importance of employing in vitro models that are more physiologically relevant and that better mimic the in vivo situation for the toxicological screening of substances. (AU) | |
| FAPESP's process: | 13/00735-0 - Development Epidermis Equivalent On New Biopolymeric Membrane |
| Grantee: | Carolina Motter Catarino |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 11/07441-7 - Development of an artificial skin platform for risk assessment |
| Grantee: | Silvya Stuchi Maria-Engler |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/14327-6 - Development of artificial skin containing glycated dermal equivalent in the assessment of efficacy and toxicity of compound anti-glycation |
| Grantee: | Silvya Stuchi Maria-Engler |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/22812-1 - DEVELOPMENT OF ARTIFICIAL SKIN CONTAINING GLYCATED DERMAL EQUIVALENT MIMICKING THE PATHOPHYSIOLOGY OF SKIN AGING AND DIABETES |
| Grantee: | Paula Comune Pennacchi |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/12682-9 - Immunosuppressive effects of UV radiation in immunocompetent epidermal equivalent |
| Grantee: | Tatiana Do Nascimento Pedrosa |
| Support Opportunities: | Scholarships in Brazil - Doctorate |