Modulating effect of low intensity pulsed ultrasound on the phenotype of inflammatory cells

Author(s)

da Silva Junior, Evaldo Moreira ^{[1, 2]} ; Mesquita-Ferrari, Raquel Agnelli ^{[1, 2]} ; Franca, Cristiane Miranda ^[1] ; Andreo, Lucas ^{[1, 2]} ; Bussadori, Sandra Kalil ^{[1, 2]} ; Santos Fernandes, Kristianne Porta ^{[1, 2]}

Affiliation

^[1] Univ Nove Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, 249 Vergueiro St, BR-01504001 Sao Paulo, SP - Brazil

^[2] Univ Nove Julho UNINOVE, Postgrad Program Rehabil Sci, 249 Vergueiro St, BR-0150400 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: BIOMEDICINE & PHARMACOTHERAPY; v. 96, p. 1147-1153, DEC 2017.
• Web of Science Citations: 2

Abstract

Aims: The purpose of this study was toclarify the effect of low intensity pulsed ultrasound (LIPUS) on the phenotype of the inflammatory infiltrate in muscle tissue following acute injury. Main methods: Forty Wistar rats were submitted to cryoinjury of the tibialis anterior muscle and only half were treated with LIPUS. After 1, 2, 3 and 7 days macrophages and neutrophils were quantified. Key findings: With one day, LIPUS led to reductions in the number of neutrophils and M1 macrophages. After two days, muscles treated with LIPUS had fewer total macrophages and M1 macrophages, but a greater number of M2 macrophages. Muscles treated with LIPUS showed fewer macrophages after three and seven days. Significance: As the permanence of cells with pro-inflammatory and anti-inflammatory action can lead to the perpetuation of inflammation with consequent tissue damage and tissue fibrosis, respectively, the ability of LIPUS to modulate the occurrence of these cells demonstrates the therapeutic potential of this resource. (AU)

• FAPESP's process: 14/12381-1 - Comparative analysis of the effect of previous laser therapy associated or not to post treatment on the modulation of inflammatory cytokines, oxidative stress and the determination of muscle fiber type after acute injury
• Grantee: Raquel Agnelli Mesquita Ferrari
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/07502-1 - Effect of laser therapy on gene expression and production of cytokines, chemokines and growth factors involved in muscle repair
• Grantee: Kristianne Porta Santos Fernandes
• Support Opportunities: Regular Research Grants