Freeze-drying of silica nanoparticles: redispersibility toward nanomedicine applications

Author(s)

Picco, Agustin S. ^[1] ; Ferreira, Larissa F. ^{[1, 2]} ; Liberato, Michelle S. ^[1] ; Mondo, Gabriela B. ^{[1, 3]} ; Cardoso, Mateus B. ^{[1, 2, 3]}

Affiliation

^[1] CNPEM, LNLS, Lab Nacl Nanotecnol LNNano, Caixa Postal 6192, BR-13083970 Campinas, SP - Brazil

^[2] Univ Fed ABC, Programa Posgrad Biotecnociencia, BR-09210580 Santo Andre - Brazil

^[3] Univ Estadualde Campinas GBM1, IQ, Caixa Postal 6154, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Nanomedicine; v. 13, n. 2, p. 179-190, JAN 2018.
• Web of Science Citations: 6

Abstract

Aim: To study freeze-drying of silica nanoparticles (SiO(2)NPs) in order to find suitable conditions to produce lyophilized powders with no aggregation after resuspension and storage. Methods: SiO(2)NPs were synthesized using a Stober-based procedure, and characterized by scanning electron microscopy, dynamic light scattering and nitrogen adsorption/desorption isotherms. SiO(2)NPs hydrodynamic diameters were compared prior and after freeze-drying in the presence/absence of carbohydrate protectants. Results: Glucose was found to be the most suitable protectant against the detrimental effects of lyophilization. The minimum concentration of carbohydrate required to effectively protect SiO(2)NPs from aggregation during freeze-drying is influenced by the nanoparticle's size and texture. Negligible aggregation was observed during storage. Conclusion: Carbohydrates can be used during SiO(2)NPs freeze-drying process to obtain redispersable solids that maintain original sizes without residual aggregation. (AU)

• FAPESP's process: 16/16905-0 - Colloidal stability and protein corona: fundamental aspects for maintaining silica nanoparticle properties in biologial media
• Grantee: Gabriela Borba Mondo
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 15/25406-5 - Organizing matter: colloids formed by association of surfactants, polymers and nanoparticles
• Grantee: Watson Loh
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/22322-2 - Functionalization of silica nanoparticles: increasing biological interaction
• Grantee: Mateus Borba Cardoso
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/21910-8 - EVOLUTION OF SILICA NANOPARTICLES IN BIOLOGICAL MEDIA: A STUDY ON PROTEIN CORONA FORMATION, AGGREGATION AND CELLULAR UPTAKE
• Grantee: Agustín Silvio Picco
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/02145-7 - Study of the interaction between proteins and functionalized silica nanoparticles
• Grantee: Larissa Fernanda Ferreira
• Support Opportunities: Scholarships in Brazil - Master