Screening of tropically derived, multi-trait plant growth- promoting rhizobacteria and evaluation of corn and soybean colonization ability

Author(s):Show less -

Batista, Bruna Durante ^[1] ; Lacava, Paulo Teixeira ^[2] ; Ferrari, Andrea ^[1] ; Teixeira-Silva, Natalia Sousa ^[1] ; Bonatelli, Maria Leticia ^[1] ; Tsui, Sarina ^[1] ; Mondin, Mateus ^[1] ; Kitajima, Elliot Watanabe ^[3] ; Pereira, Jose Odair ^[4] ; Azevedo, Joao Ludo ^[1] ; Quecine, Maria Carolina ^[1]

Affiliation

^[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Genet, 11 Padua Dias Av, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Morphol & Pathol, Ctr Biol & Hlth Sci, Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Plant Pathol & Hematol, Piracicaba, SP - Brazil

^[4] Univ Fed Amazonas, Manaus, Amazonas - Brazil

• Document type: Journal article
• Source: MICROBIOLOGICAL RESEARCH; v. 206, p. 33-42, 2018.
• Web of Science Citations: 7

Abstract

The present study assessed the plant growth-promoting (PGP) traits and diversity of culturable rhizobacteria associated with guarana (Paullinia cupana), a typical tropical plant. Ninety-six bacteria were isolated, subjected to biochemical tests, and identified by partial or total 16S rDNA sequencing. Proteobacteria and Firmicutes were the dominant rhizospheric phyla found, and Burkholderia and Bacillus were the most abundant genera. Thirteen strains exhibited the four PGP traits evaluated, and most of them belonged to the genus Burkholderia. Two multi trait PGP strains, RZ2MS9 (Bacillus sp.) and RZ2MS16 (Burkholderia ambifaria), expressively promoted corn and soybean growth under greenhouse conditions. Compared to the non-inoculated control, increases in corn root dry weight of 247.8 and 136.9% were obtained with RZ2MS9 and RZ2MS16 inoculation, respectively, at 60 days after seeding. The dry weights of corn and soybean shoots were significantly higher than those of non-inoculated plants, showing increases of more than 47% for both strains and crops. However, soybean root dry weight did not increased after bacterial inoculation with either strain. The colonization behavior of RZ2MS16 was assessed using GFP-labeling combined with fluorescence microscopy and a cultivation-based approach for quantification. RZ2MS16:gfp was able to colonize the roots and shoots of corn and soybean, revealing an endophytic behavior. (AU)

• FAPESP's process: 11/05204-8 - Plant growth promotion by rhizobacteria of guarana.
• Grantee: Bruna Durante Batista
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 10/14382-4 - Culturable and nonculturable endophytic microorganisms of guarana and the anthracnose control.
• Grantee: Maria Leticia Bonatelli
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 15/01188-9 - Phenotypic and genome-wide association studies of the interaction between Burkholderia ambifaria RZ2MS16, a plant growth promoter rhizobacteria, and the tropical maize
• Grantee: João Lúcio de Azevedo
• Support type: Regular Research Grants
• FAPESP's process: 10/15192-4 - Study of the diversity of culturable endophytic bacteria associated with guarana [Paullinia cupana (Mart.) Ducke.] for the control of anthracnose
• Grantee: Sarina Tsui
• Support type: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 14/05420-0 - Monitoring of interaction between plant growth promoter rhizobacterium and rhizobium in soybeans [Glycine max (L.) Merr.]
• Grantee: Andréa Ferrari
• Support type: Scholarships in Brazil - Scientific Initiation