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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Transport of cowpea bean derived peptides and their modulator effects on mRNA expression of cholesterol-related genes in Caco-2 and HepG2 cells

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Marques, Marcelo Rodrigues [1, 2] ; Cerda, Alvaro [3] ; Fontanari, Gustavo Guadagnucci [1] ; Pimenta, Daniel Carvalho [4] ; Soares-Freitas, Rosana Manolio [1] ; Hirata, Mario Hiroyuki [5] ; Crespo Hirata, Rosario Dominguez [5] ; Gomes Areas, Jose Alfredo [1]
Total Authors: 8
[1] Univ Sao Paulo, Sch Publ Hlth, Nutr Dept, Lab Biochem & Funct Properties Food, Sao Paulo - Brazil
[2] Inst Fed Maranhao IFMA, Ave Tecnol 215, BR-65365000 Ze Doca, MA - Brazil
[3] Univ La Frantera, CEMT BIOREN, Ctr Excelencia Med Translac, Dept Ciencias Basicas, Temuco - Chile
[4] Inst Butantan, Lab Biochem & Biophys, Sao Paulo - Brazil
[5] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Food Research International; v. 107, p. 165-171, MAY 2018.
Web of Science Citations: 1

This work studied the cell transport of peptidase-generated peptides from cowpea bean proteins and their effects on mRNA expression of cholesterol-related genes in intestinal and liver cells. The <= 3 kDa hydrolysate was obtained and incubated with Caco-2 intestinal cells using Transwell center dot plates. HepG2 liver cells were incubated with synthetic analogues of peptides (MELNAVSVVHS and MELNAVSVVSH) identified by ``de novo{''} peptide sequencing in the Caco-2 monolayer permeates. The mRNA expression of NPC1L1, ABCA1 and ABCG1 was measured in Caco-2 cells, in the presence or absence of <= 3 kDa hydrolysate and the expression of HMGCR, SREBP2, LXRa, AMPK1, was determined in the HepG2 cells in the presence or absence of synthetic peptides. Exposure of Caco-2 cells to cowpea <= 3 kDa hydrolysate (2.5 and 5 mg/mL) increased ABCG1 expression at 6 h and 12 h. SREBP2, HMGCR and LDLR mRNA levels were reduced in HepG2 cells after 24 h of treatment with MELNAVSVVHS peptide (50 mu M and 100 mu M). These results suggest that MELNAVSVVHS peptide is able to cross intestinal barrier and to modulate genes involved in cholesterol homeostasis. (AU)

FAPESP's process: 13/09304-2 - Modulating of cholesterol biosynthesis and absorption by peptides from cowpea bean (Vigna unguiculata L. Walp)
Grantee:Marcelo Rodrigues Marques
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/15900-4 - Bioavailability of peptides from proteins of lupine, cowpea and amaranth, by permeability through Caco-2 cells and alterations in gene expression of the transporters associated with intestinal cholesterol absorption
Grantee:José Alfredo Gomes Arêas
Support type: Regular Research Grants