Transport of cowpea bean derived peptides and their modulator effects on mRNA expression of cholesterol-related genes in Caco-2 and HepG2 cells

Author(s)

Marques, Marcelo Rodrigues ^{[1, 2]} ; Cerda, Alvaro ^[3] ; Fontanari, Gustavo Guadagnucci ^[1] ; Pimenta, Daniel Carvalho ^[4] ; Soares-Freitas, Rosana Manolio ^[1] ; Hirata, Mario Hiroyuki ^[5] ; Crespo Hirata, Rosario Dominguez ^[5] ; Gomes Areas, Jose Alfredo ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Publ Hlth, Nutr Dept, Lab Biochem & Funct Properties Food, Sao Paulo - Brazil

^[2] Inst Fed Maranhao IFMA, Ave Tecnol 215, BR-65365000 Ze Doca, MA - Brazil

^[3] Univ La Frantera, CEMT BIOREN, Ctr Excelencia Med Translac, Dept Ciencias Basicas, Temuco - Chile

^[4] Inst Butantan, Lab Biochem & Biophys, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil

• Document type: Journal article
• Source: Food Research International; v. 107, p. 165-171, MAY 2018.
• Web of Science Citations: 1

Abstract

This work studied the cell transport of peptidase-generated peptides from cowpea bean proteins and their effects on mRNA expression of cholesterol-related genes in intestinal and liver cells. The <= 3 kDa hydrolysate was obtained and incubated with Caco-2 intestinal cells using Transwell center dot plates. HepG2 liver cells were incubated with synthetic analogues of peptides (MELNAVSVVHS and MELNAVSVVSH) identified by ``de novo{''} peptide sequencing in the Caco-2 monolayer permeates. The mRNA expression of NPC1L1, ABCA1 and ABCG1 was measured in Caco-2 cells, in the presence or absence of <= 3 kDa hydrolysate and the expression of HMGCR, SREBP2, LXRa, AMPK1, was determined in the HepG2 cells in the presence or absence of synthetic peptides. Exposure of Caco-2 cells to cowpea <= 3 kDa hydrolysate (2.5 and 5 mg/mL) increased ABCG1 expression at 6 h and 12 h. SREBP2, HMGCR and LDLR mRNA levels were reduced in HepG2 cells after 24 h of treatment with MELNAVSVVHS peptide (50 mu M and 100 mu M). These results suggest that MELNAVSVVHS peptide is able to cross intestinal barrier and to modulate genes involved in cholesterol homeostasis. (AU)

• FAPESP's process: 12/15900-4 - Bioavailability of peptides from proteins of lupine, cowpea and amaranth, by permeability through Caco-2 cells and alterations in gene expression of the transporters associated with intestinal cholesterol absorption
• Grantee: José Alfredo Gomes Arêas
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/09304-2 - Modulating of cholesterol biosynthesis and absorption by peptides from cowpea bean (Vigna unguiculata L. Walp)
• Grantee: Marcelo Rodrigues Marques
• Support Opportunities: Scholarships in Brazil - Doctorate