Effect of non-thermal atmospheric plasma on the dentin-surface topography and composition and on the bond strength of a universal adhesive

Author(s)

Ayres, Ana P. ^[1] ; Bonvent, Jean J. ^[2] ; Mogilevych, Borys ^[3] ; Soares, Luis E. S. ^[4] ; Martin, Airton A. ^[3] ; Ambrosano, Glaucia M. ^[5] ; Nascimento, Fabio D. ^[6] ; Van Meerbeek, Bart ^{[7, 8]} ; Giannini, Marcelo ^[1]

Affiliation

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Dent Mat, Piracicaba - Brazil

^[2] Fed Univ ABC, Dept Ctr Nat & Human Sci, Santo Andre - Brazil

^[3] Univ Vale Paraiba, Res & Dev Inst IP&D, Lab Biomed Vibrat Spect LEVB, Sao Jose Dos Campos - Brazil

^[4] Univ Vale Paraiba, Res & Dev Inst IP&D, Lab Dent & Appl Mat LDAM, Sao Jose Dos Campos - Brazil

^[5] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Stat, Piracicaba - Brazil

^[6] Univ Mogi das Cruzes, Interdisciplinary Ctr Biochem Invest, Mogi Das Cruzes - Brazil

^[7] Katholieke Univ Leuven, BIOMAT, Dept Oral Hlth Sci, Leuven - Belgium

^[8] Katholieke Univ Leuven, Univ Hosp Leuven, Leuven - Belgium

• Document type: Journal article
• Source: European Journal of Oral Sciences; v. 126, n. 1, p. 53-65, FEB 2018.
• Web of Science Citations: 3

Abstract

This study investigated the effect of application of non-thermal atmospheric plasma (NTAP) on the topography and composition of the dentin surface, as well as the microtensile bond strength (TBS) of a universal adhesive to NTAP-treated dentin. Exposed flat dentin surfaces from human third molars were either treated with NTAP for 10 and 30s or untreated (control). The dentin-surface topography and chemical composition were characterized by atomic force microscopy (n=3) and Raman confocal spectroscopy (n=5), respectively. The TBS (n=8) of Scotchbond Universal to dentin was determined after storage for 24h and 1yr, either by direct water exposure or under simulated pulpal pressure. In-situ zymography was used to evaluate the influence of NTAP on the dentin-enzymatic activity. Non-thermal atmospheric plasma produced no remarkable topographical or chemical alterations at the dentin surface; only the amount of phosphate decreased following 10s of treatment with NTAP. After 1yr of direct water exposure, the TBS of NTAP-treated specimens did not differ statistically significantly from that of untreated controls, whereas simulated pulpal pressure-aging resulted in a significantly higher TBS for NTAP-treated dentin. The dentin-enzymatic activity appeared to be treatment-dependent, but the untreated controls showed more intense fluorescence within the hybrid layer. Scotchbond Universal maintained its TBS strength after 1yr of direct water exposure and simulated pulpal pressure, although remarkable statistical differences between treatments were observed depending on the aging condition. (AU)

• FAPESP's process: 13/15952-7 - Effect of argon plasma on the dentinal surface and the adhesion of restorative material
• Grantee: Ana Paula Almeida Ayres
• Support Opportunities: Scholarships in Brazil - Doctorate