In vitro and in vivo biological performance of porous Ti alloys prepared by powder metallurgy

Author(s):Show less -

do Prado, Renata Falchete ^[1] ; Esteves, Gabriela Campos ^[1] ; De Souza Santos, Evelyn Luzia ^[1] ; Griti Bueno, Daiane Acacia ^[1] ; Alves Cairo, Carlos Alberto ^[2] ; Oliveira De Vasconcellos, Luis Gustavo ^[3] ; Sagnori, Renata Silveira ^[4] ; Pereira Tessarin, Fernanda Bastos ^[5] ; Oliveira, Felipe Eduardo ^[1] ; De Oliveira, Luciane Dias ^[1] ; Lima Villaca-Carvalho, Maria Fernanda ^[1] ; Rodrigues Henriques, Vinicius Andre ^[2] ; Carvalho, Yasmin Rodarte ^[1] ; Reis De Vasconcellos, Luana Marotta ^[1]

Affiliation

^[1] Sao Paulo State Univ, UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil

^[2] Praca Mal Ar Eduardo Gomes, Air & Space Inst, Div Mat, Sao Jose Dos Campos, SP - Brazil

^[3] Sao Paulo State Univ, UNESP, Inst Sci & Technol, Dept Prosthodont & Dent Mat, Sao Jose Dos Campos, SP - Brazil

^[4] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Oral Diag, Piracicaba, SP - Brazil

^[5] Sao Paulo State Univ, UNESP, Inst Sci & Technol, Dept Restorat Dent, Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: PLoS One; v. 13, n. 5 MAY 17 2018.
• Web of Science Citations: 4

Abstract

Titanium (Ti) and Ti-6 Aiuminium-4 Vanadium alloys are the most common materials in implants composition but beta type alloys are promising biomaterials because they present better mechanical properties. Besides the composition of biomaterial, many factors influence the performance of the biomaterial. For example, porous surface may modify the functional cellular response and accelerate osseointegration. This paper presents in vitro and in vivo evaluations of powder metallurgy-processed porous samples composed by different titanium alloys and pure Ti, aiming to show their potential for biomedical applications. The porous surfaces samples were produced with different designs to in vitro and in vivo tests. Samples were characterized with scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and elastic modulus analyses. Osteogenic cells from newborn rat calvaria were plated on discs of different materials: G1-commercially pure Ti group (CpTi); G2-Ti-6AI-4V alloy; G3-Ti-13 Niobium-13 Zirconium alloy; G4-Ti-35 Niobium alloy; G5-Ti-35 Niobium-7 Zirconium-5 Tantalum alloy. Cell adhesion and viability, total protein content, alkaline phosphatase activity, mineralization nodules and gene expression (alkaline phosphatase, Runx-2, osteocalcin and osteopontin) were assessed. After 2 and 4 weeks of implantation in rabbit tibia, bone ingrowth was analyzed using micro-computed tomography (mu CT). EDS analysis confirmed the material production of each group. Metallographic and SEM analysis revealed interconnected pores, with mean pore size of 99,5 mu m and mean porosity of 42%, without significant difference among the groups (p > 0.05). The elastic modulus values did not exhibit difference among the groups (p > 0.05). Experimental alloys demonstrated better results than CpTi and Ti-6AI-4V, in gene expression and cytokines analysis, especially in early experimental periods. In conclusion, our data suggests that the experimental alloys can be used for biomedical application since they contributed to excellent cellular behavior and osseointegration besides presenting lower elastic modulus. (AU)

• FAPESP's process: 12/20311-8 - In vivo comparison of biocompatibility of porous surface samples made with different titanium alloys
• Grantee: Luana Marotta Reis de Vasconcellos
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/23662-3 - Caracterization and in vitro study of the biocompatibility of titanium alloys for biomedical application
• Grantee: Yasmin Rodarte Carvalho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/15342-9 - In vivo comparison of biocompatibility of porous surface samples made with different titanium alloys
• Grantee: Evelyn Luzia de Souza Santos
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/13338-7 - In vitro study of biocompatibility of porous titanium alloys samples for biomedical application
• Grantee: Fernanda Bastos Pereira Tessarin
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/13324-6 - In vitro study of biocompatibility of porous titanium alloys samples for biomedical application
• Grantee: Renata Silveira Sagnori
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/22158-2 - Comparative in vivo study of bone ingrowth in porous titanium alloys samples processed via powder metallurgy
• Grantee: Daiane Acacia Griti Bueno
• Support Opportunities: Scholarships in Brazil - Scientific Initiation