Chemical Composition and In vitro Anthelmintic Activity of Extracts of Tagetes patula Against a Multidrug-Resistant Isolate of Haemonchus contortus

Author(s)

Sanches Politi, Flavio Augusto ^[1] ; Souza Junior, Amauri Alves ^[1] ; Fantatto, Rafaela Regina ^[2] ; Linhari Rodrigues Pietro, Rosemeire Cristina ^[2] ; Barioni Junior, Waldomiro ^[3] ; Rabelo, Marcio Dias ^[3] ; Bizzo, Humberto Ribeiro ^[4] ; de Souza Chagas, Ana Carolina ^[3] ; Furlan, Maysa ^[1]

Affiliation

^[1] UNESP Univ Estadual Paulista, Inst Chem, Dept Organ Chem, Rua Prof Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Drugs & Med, Rodovia Araraquara Jau Km 01 S-N, BR-14800903 Araraquara, SP - Brazil

^[3] Embrapa Pecuaria Sudeste, Empresa Brasileira Pesquisa Agr, Rodovia Washington Luiz Km 234 S-N, BR-13560970 Sao Carlos, SP - Brazil

^[4] Embrapa Agroind Alimentos, Empresa Brasileira Pesquisa Agr, Ave Amer 29501, BR-23020470 Rio De Janeiro, RJ - Brazil

• Document type: Journal article
• Source: CHEMISTRY & BIODIVERSITY; v. 15, n. 2 FEB 2018.
• Web of Science Citations: 3

Abstract

Sheep breeding has suffered economic losses due to parasitism by gastrointestinal nematodes, particularly Haemonchus contortus. The use of natural products, specifically Tagetes patula, has been suggested as an alternative method of combatting this issue. Chemical analyses of the extracts of this species described in the literature report the presence of important classes of secondary metabolites such as thiophenes, flavonoids, alkaloids, and benzofurans, some of which were identified and isolated in this study. The aim of this work was to test the effect of the essential oil (EO) and the ethanolic extract of the aerial parts (Tp(EtOH)) of T. patula on eggs and larvae of H. contortus, through an egg hatch test (EHT) and a larval development test (LDT). In the EHT, the EO showed 100% inhibition at 0.75 mg mL(-1) (LC50 = 0.0780 mg mL(-1)), and the Tp(EtOH) showed 100% inhibition at 100 mg mL(-1) (LC50 = 12.8 mg mL(-1)). In the LDT, the EO showed 100% inhibition at 0.375 mg mL(-1) (LC50 = 0.0400 mg mL(-1)), and the Tp(EtOH) showed 100% inhibition at 1.56 mg mL(-1) (LC50 = 0.340 mg mL(-1)). Compared to available literature data, the results presented here suggest that the crude extracts of T. patula have substantial potential for controlling this nematode by interrupting its life cycle and/or preventing it from reaching the infective stage. (AU)

• FAPESP's process: 13/03493-8 - Potential of crude extracts, essential oil and pure compounds obtained from Tagetes patula L. (Asteraceae) as botanical biopesticide against Rhipicephalus (Boophilus) microplus, Haemonchus contortus and Cimex lectularis
• Grantee: Flávio Augusto Sanches Politi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
• Grantee: Glaucius Oliva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC