Effects of Glutamine and Alanine Supplementation on Central Fatigue Markers in Rats Submitted to Resistance Training

Author(s)

Coqueiro, Audrey Yule ^[1] ; Raizel, Raquel ^[1] ; Bonvini, Andrea ^[1] ; Hypolito, Thais ^[2] ; Godois, Allan da Mata ^[3] ; Rocha Pereira, Jessica Ramos ^[1] ; de Oliveira Garcia, Amanda Beatriz ^[1] ; Bittencourt Lara, Rafael de Souza ^[1] ; Rogero, Marcelo Macedo ^[2] ; Tirapegui, Julio ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, Ave Prof Lineu Prestes 580, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Publ Hlth, Dept Nutr, Ave Doutor Arnaldo 715, BR-01246904 Sao Paulo, SP - Brazil

^[3] Univ Fed Mato Grosso, Fac Nutr, Ave Fernando Correa 2367, BR-78060900 Cuiaba, MT - Brazil

• Document type: Journal article
• Source: NUTRIENTS; v. 10, n. 2 FEB 2018.
• Web of Science Citations: 6

Abstract

Recent evidence suggests that increased brain serotonin synthesis impairs performance in high-intensity intermittent exercise and specific amino acids may modulate this condition, delaying fatigue. This study investigated the effects of glutamine and alanine supplementation on central fatigue markers in rats submitted to resistance training (RT). Wistar rats were distributed in: sedentary (SED), trained (CON), trained and supplemented with alanine (ALA), glutamine and alanine in their free form (G + A), or as dipeptide (DIP). Trained groups underwent a ladder-climbing exercise for eight weeks, with progressive loads. In the last 21 days, supplementations were offered in water with a 4% concentration. Albeit without statistically significance difference, RT decreased liver glycogen, and enhanced the concentrations of plasma glucose, free fatty acids (FFA), hypothalamic serotonin, and ammonia in muscle and the liver. Amino acids affected fatigue parameters depending on the supplementation form. G + A prevented the muscle ammonia increase by RT, whereas ALA and DIP augmented ammonia and glycogen concentrations in muscle. DIP also increased liver ammonia. ALA and G + A reduced plasma FFA, whereas DIP increased this parameter, free tryptophan/total tryptophan ratio, hypothalamic serotonin, and the serotonin/dopamine ratio. The supplementations did not affect physical performance. In conclusion, glutamine and alanine may improve or impair central fatigue markers depending on their supplementation form. (AU)

• FAPESP's process: 16/04910-0 - Effect of chronic supplementation with L-glutamine and L-alanine, in free form or as dipeptide, on parameters associated with fatigue in rats subjected to resistance training.
• Grantee: Julio Orlando Tirapegui Toledo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/22789-3 - Effect of chronic oral supplementation with L-glutamine and L-alanine, in their free forms or as dipeptide, on parameters associated with fatigue in rats submitted to resistance exercise
• Grantee: Audrey Yule Coqueiro
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)