Analysis of antioxidant, cytotoxic and mutagenic potential of Agave sisalana Perrine extracts using Vero cells, human lymphocytes and mice polychromatic erythrocytes

Author(s)

Araldi, Rodrigo Pinheiro ^{[1, 2]} ; dos Santos, Maristela Oliveira ^[3] ; Barbon, Fabiane Faria ^[3] ; Manjerona, Bruna Aparecida ^[3] ; Meirelles, Bruno Rosa ^[3] ; Neto, Pedro de Oliva ^[3] ; da Silva Junior, Pedro Ismael ^[4] ; dos Santos, Lucineia ^[3] ; Cherici Camargo, Isabel Cristina ^[3] ; de Souza, Edislane Barreiros ^[3]

Affiliation

^[1] Butantan Inst, Genet Lab, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Biomed Sci Inst, Biotechnol Interunit Post Grad Program, Sao Paulo, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Sci Humanities & Languages, Dept Biotechnol, Assis, SP - Brazil

^[4] Butantan Inst, Ctr Toxins Immune Response & Cell Signaling CeTIC, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: BIOMEDICINE & PHARMACOTHERAPY; v. 98, p. 873-885, FEB 2018.
• Web of Science Citations: 1

Abstract

Brazilian Northeast is the world's largest producer of Agave sisalana Perrine for the supply of the sisal fiber. About 95% of plant biomass, which comprise the mucilage and sisal juice, is considered a waste residual is discarded in the soil. However, the sisal juice is rich in steroidal saponins, which exhibits different pharmacological properties. Despite this, natural products are not necessarily safe. Based on this, this study analyzed the antioxidant, cytotoxic and mutagenic potential of three extracts derived from acid hydrolysis (AHAS), dried precipitate (DPAS) and hexanic of A. sisalana (HAS). These analyses were performed by in vitro and in vivo methods, using Vero cells, human lymphocytes and mice. Results showed that AHAS 50 and 100 can be considered a useful antineoplastic candidate due to their antioxidant and cytotoxic activity, with no genotoxic/clastogenic potential in Vero cells and mice. Although the comet assay in human lymphocytes has showed that the AHAS 25, AHAS 50 and AHAS 100 can lead to DNA breaks, these extracts did not promote DNA damages in mice bone marrow. Considering the different mutagenic responses obtained with the different methods employed, this study suggest that the metabolizing pathways can produce by-products harmful to health. For this reason, it is mandatory to analyze the mutagenic potential by both in vitro and in vivo techniques, using cells derived from different species and origins. (AU)

• FAPESP's process: 14/20617-5 - Study of the action of bovine papillomavirus (BPV) in epithelial-mesenchymal transition
• Grantee: Rodrigo Pinheiro Araldi
• Support Opportunities: Scholarships in Brazil - Doctorate