Cytotoxic and genotoxic potential study of Copaifera lucens in extract cells of mammals in vitro and in vivo

• Grant number: 15/12372-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): February 01, 2016
• Effective date (End): December 31, 2016
• Field of knowledge: Health Sciences - Pharmacy - Pharmacognosy
• Principal Investigator: Juliana Marques Senedese Dias
• Grantee: Larissa Daniela Ribeiro de Souza
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species, AP.TEM

Abstract

The use of plants for medicinal purposes has been practiced since ancient times and remains widespread to this day. Based on popular culture passed from generation to generation, many people have made use of plant species for the prevention or treatment of diseases. The trees of the genus Copaifera have been widely studied. This genus comprises about 72 species of which 20 occur in Brazil and is commonly found in the Southeast, Midwest and Amazon. The species of this genus have various biological properties such as anti-inflammatory, antibacterial and antifungal. The Copaifera lucens species is used for healing wounds, treatment of stroke, sore throat pain, and vision problems. Faced with various biological properties of the species Copaifera genus and the species Copaifera lucens, this study aims to evaluate the cytotoxic and genotoxic potential of the extract of the leaves of Copaifera lucens in mammalian cells in vitro and in vivo. In vitro test system, the cytotoxicity is evaluated by the clonogenic assay efficiency. The micronucleus test in Chinese hamster lung fibroblasts (V79 cells) and bone marrow Swiss mice will be used to study the possible genotoxicity extract. Cytotoxicity will be evaluated by cell division rates in vitro test system and the ratio of polychromatic erythrocytes and normochromatic erythrocytes in vivo test system. The results of this study will contribute to the knowledge of biological properties of species of Copaifera poorly studied.