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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Novel Conducting and Biodegradable Copolymers with Noncytotoxic Properties toward Embryonic Stem Cells

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Author(s):
da Silva, Arua C. [1] ; Semeano, Ana Teresa S. [2] ; Dourado, Andre H. B. [1] ; Ulrich, Henning [2] ; Cordoba de Torresi, I, Susana
Total Authors: 5
Affiliation:
[1] I, Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ACS OMEGA; v. 3, n. 5, p. 5593-5604, MAY 2018.
Web of Science Citations: 5
Abstract

Electroactive biomaterials that are easily processed as scaffolds with good biocompatibility for tissue regeneration are difficult to design. Herein, the synthesis and characterization of a variety of novel electroactive, biodegradable biomaterials based on poly(3,4-ethylenedioxythiphene) copolymerized with poly(D, L lactic acid) (PEDOT-co-PDLLA) are presented. These copolymers were obtained using (2,3-dihydrothieno{[}3,4-b]{[}1,4]dioxin-2-yl) methanol (EDOT-OH) as an initiator in a lactide ring-opening polymerization reaction, resulting in EDOT-PDLLA macromonomer. Conducting PEDOT-co-PDLLA copolymers (in three different proportions) were achieved by chemical copolymerization with 3,4-ethylenedioxythiophene (EDOT) monomers and persulfate oxidant. The PEDOT-co-PDLLA copolymers were structurally characterized by H-1 NMR and Fourier transform infrared spectroscopy. Cyclic voltammetry confirmed the electroactive character of the materials, and conductivity measurements were performed via electrochemical impedance spectroscopy. In vitro biodegradability was evaluated using proteinase K over 35 days, showing 29-46% (w/w) biodegradation. Noncytotoxicity was assessed by adhesion, migration, and proliferation assays using embryonic stem cells (E14.tg2a); excellent neuronal differentiation was observed. These novel electroactive and biodegradable PEDOT-co-PDLLA copolymers present surface chemistry and charge density properties that make them potentially useful as scaffold materials in different fields of applications, especially for neuronal tissue engineering. (AU)

FAPESP's process: 14/09353-6 - Biomaterials based on conducting biodegradable and biocompatible polymers
Grantee:Aruã Clayton da Silva
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 13/25592-8 - Study of L-cysteine and L-cystine adsorption on platinum electrodes
Grantee:André Henrique Baraldi Dourado
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 12/50880-4 - Stem cells: from basic studies of kinin and purinergic receptor roles towards therapeutical applications
Grantee:Alexander Henning Ulrich
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/26308-7 - Optimization of the physicochemical properties of nano -structured materials for applications in molecular recognition, catalysis and energy conversion/storage
Grantee:Roberto Manuel Torresi
Support Opportunities: Research Projects - Thematic Grants