Metabolic routes involved in the removal of linear alkylbenzene sulfonate (LAS) employing linear alcohol ethoxylated and ethanol as co-substrates in enlarged scale fluidized bed reactor

Author(s)

Motteran, Fabricio ^[1] ; Nadai, Bianca Marques ^[1] ; Braga, Juliana Kawanishi ^[1] ; Silva, Edson Luiz ^[2] ; Amancio Varesche, Maria Bernadete ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Engn Sao Carlos, Dept Hydraul & Sanit, Ave Trabalhador Saocarlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Chem Engn, Rod Washington Luiz, Km 235, SP 310, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Science of The Total Environment; v. 640, p. 1411-1423, NOV 1 2018.
• Web of Science Citations: 3

Abstract

In this study, the microbial community characterization and metabolic pathway identification involved in the linear alkylbenzene sulfonated (LAS) degradation from commercial laundry wastewater in a fluidized bed reactor (FBR) on an increased scale were performed using the Illumina MiSeq platform. Ethanol and non-ionic surfactant (LAE, Genapol C-100) were used as co-substrates. The FBR was operated in five operational phases: (I) synthetic substrate for inoculation; (II) 7.9 +/- 4.7 mg/L LAS and 11.7 +/- 6.9 mg/L LAE; (III) 19.4 +/- 12.9 mg/L LAS, 19.6 +/- 9.2 mg/L LAE and 205 mg/L ethanol; (IV) 25.9 +/- 11 mg/L LAS, 19.5 +/- 9.1 mg/L LAE and 205 mg/L ethanol and (V) 43.9 +/- 18 mg/L LAS, 25 +/- 9.8 mg/L LAE and 205 mg/L ethanol. At all operation phases, organic matter was removed from 40.4 to 85.1% and LAS removal was from 24.7 to 56%. Sulfate-reducing bacteria (SRB) were identified in the biofilm of FBR in all operational phases. Although the LAS promoted a toxic effect on the microbiota, this effect can be reduced when using biodegradable co-substrates, such as ethanol and LAE, which was observed in Phase IV. In this phase, there was a greater microbial diversity (Shannon index) and higher microorganism richness (Chao 1 index), both for the Domain Bacteria, and for the Domain Archaea. (c) 2018 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 13/19025-3 - INCREASING SCALE OF ANAEROBIC FLUIDIZED BED REACTOR FOR REMOVAL OF ANIONIC AND NONIONIC SURFACTANTS IN COMMERCIAL LAUNDRY WASTEWATER
• Grantee: Fabricio Motteran
• Support Opportunities: Scholarships in Brazil - Post-Doctoral