Steroidal hormone and morphological responses in the prostate anterior lobe in different cancer grades after Celecoxib and Goniothalamin treatments in TRAMP mice

Author(s)

Silva, Rafael Sauce ^[1] ; Kido, Larissa Akemi ^[1] ; Montico, Fabio ^[1] ; Vendramini-Costa, Debora Barbosa ^[2] ; Pilli, Ronaldo Aloise ^[3] ; Alves Cagnon, Valeria Helena ^[1]

Affiliation

^[1] Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, POB 6109, BR-13083865 Campinas, SP - Brazil

^[2] Fox Chase Ctr, Canc Biol Program, Philadelphia, PA - USA

^[3] Univ Campinas UNICAMP, Inst Chem, Dept Organ Chem, Campinas, SP - Brazil

• Document type: Journal article
• Source: Cell Biology International; v. 42, n. 8, p. 1006-1020, AUG 2018.
• Web of Science Citations: 3

Abstract

Prostate cancer is the second most diagnosed cancer in the world, and alternative methods to prevent and treat different lesion grades need to be evaluated. The objective was to evaluate the morphological, hormonal, and inflammatory responses in the prostate anterior lobe in transgenic adenocarcinoma of the mouse prostate (TRAMP), following Celecoxib and Goniothalamin (GTN) treatments. All animals were treated for 4 weeks, from 8 weeks of age and euthanized either immediately after treatment (12-week-old mice: immediate response) or later (22-week-old mice: late response). The results showed a significant increase of high-grade prostatic intraepithelial neoplasia (HGPIN) and well-differentiated adenocarcinoma (WDA), according to the age in the control groups. Celecoxib treatment decreased the WDA incidence in the late response group. GTN led to a significant healthy tissue increase, and an LGPIN and HGPIN decrease in the immediate response group. In the late response group, GTN led to healthy area increase and there was no occurrence of WDA. AR and ER immunoexpressions were reduced by both treatments in the immediate response groups. However, only GTN was able to decrease the ER level in the late response group. Regarding COX-2 immunoreactivity, both treatments reduced the frequency of this enzyme. We can conclude that the prostate anterior lobe is a good model to study prostate cancer, considering its slow progression. Both treatments led to cancer delay in the prostate anterior lobe. However, GTN pointed towards a better treatment spectrum in the signaling pathways in the prostate microenvironment, particularly in ER. (AU)

• FAPESP's process: 13/23049-5 - Prostatic lesions, inflammation and aging: effects of anti-inflammatory therapies in transgenic adenocarcinoma of the mouse prostate model and in senile FVB mice
• Grantee: Valéria Helena Alves Cagnon Quitete
• Support type: Regular Research Grants