Ethanol generation, oxidation and energy production in a cooperative bioelectrochemical system

Author(s)

Pagnoncelli, Kamila C. ^[1] ; Pereira, Andressa R. ^[1] ; Sedenho, Graziela C. ^[1] ; Bertaglia, Thiago ^[1] ; Crespilho, Frank N. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Chem, BR-13560970 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: BIOELECTROCHEMISTRY; v. 122, p. 11-25, AUG 2018.
• Web of Science Citations: 0

Abstract

Integrating in situ biofuel production and energy conversion into a single system ensures the production of more robust networks as well as more renewable technologies. For this purpose, identifying and developing new biocatalysts is crucial. Herein, is reported a bioelectrochemical system consisting of alcohol dehydrogenase (ADH) and Saccharomyces cerevisiae, wherein both function cooperatively for ethanol production and its bioelectrochemical oxidation. Here, it is shown that it is possible to produce ethanol and use it as a biofuel in a tandem manner. The strategy is to employ flexible carbon fibres (FCF) electrode that could adsorb both the enzyme and the yeast cells. Glucose is used as a substrate for the yeast for the production of ethanol, while the enzyme is used to catalyse the oxidation of ethanol to acetaldehyde. Regarding the generation of reliable electricity based on electrochemical systems, the biosystem proposed in this study operates at a low temperature and ethanol production is proportional to the generated current. With further optimisation of electrode design, we envision the use of the cooperative biofuel cell for energy conversion and management of organic compounds. (C) 2018 Elsevier B.V. All tights reserved. (AU)

• FAPESP's process: 15/16672-3 - Development of High Performance Bioelectrodes for Application in Miniaturized Biofuel Cell
• Grantee: Frank Nelson Crespilho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/19908-2 - Bioelectrochemical study of the deglycosylated glucose oxidase enzyme for application in biofuel cells
• Grantee: Andressa Ribeiro Pereira
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 15/22973-6 - Development of Microbial Fuel Biocells for Bioproduction and Oxidation of Ethanol
• Grantee: Graziela Cristina Sedenho
• Support Opportunities: Scholarships in Brazil - Doctorate