Influence of expression of UCP3, PLIN1 and PPARG2 on the oxidation of substrates after hypocaloric dietary intervention

Author(s):Show less -

de Oliveira, Cristiana Cortes ^[1] ; Nicoletti, Carolina Ferreira ^[1] ; de Souza Pinhel, Marcela Augusta ^[1] ; Parenti de Oliveira, Bruno Affonso ^[1] ; Gomes Quinhoneiro, Driele Cristina ^[1] ; Noronha, Natalia Yumi ^[1] ; Fassini, Priscila Giacomo ^[1] ; Marchini, Julio Sergio ^[1] ; da Silva Junior, Wilson Arm ^[1] ; Salgado Junior, Wilson ^[2] ; Nonino, Carla Barbosa ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Internal Med, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Surg & Anat, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Clinical Nutrition; v. 37, n. 4, p. 1383-1388, AUG 2018.
• Web of Science Citations: 1

Abstract

Background \& aims: In addition to environmental and psychosocial factors, it is known that genetic factors can also influence the regulation of energy metabolism, body composition and determination of excess weight. The objective of this study was to evaluate the influence of UCP3, PLIN1 and PPARG2 genes on the substrates oxidation in women with grade III obesity after hypocaloric dietary intervention. Subjects/methods: This is a longitudinal study with 21 women, divided into two groups: Intervention Group (G1): 11 obese women (Body Mass Index (BMI) >= 40 kg/m(2)), and Control Group (G2): 10 eutrophic women (BMI between 18.5 kg/m(2) and 24.9 kg/m(2)). Weight (kg), height (m), BMI (kg/m(2)), substrate oxidation (by Indirect Calorimetry) and abdominal subcutaneous adipose tissue were collected before and after the intervention. For the dietary intervention, the patients were hospitalized for 6 weeks receiving 1200 kcal/day. Results: There was a significant weight loss (8.4 +/- 4.3 kg - 5.2 +/- 1.8%) and reduction of UCP3 expression after hypocaloric dietary intervention. There was a positive correlation between carbohydrate oxidation and UCP3 (r = 0.609; p = 0.04), PLIN1 (r = 0.882; p = 0.00) and PPARG2 (r = 0.791; p = 0.00) expression before dietary intervention and with UCP3 (r = 0.682; p = 0.02) and PLIN1 (r = 0.745; p = 0.00) genes after 6 weeks of intervention. There was a negative correlation between lipid oxidation and PLIN1 (r = -0.755; p = 0.00) and PPARG2 (r = 0.664; p = 0.02) expression before dietary intervention and negative correlation with PLIN1 (r = 0.730; p = 0.02) expression after 6 weeks of hypocaloric diet. Conclusion: Hypocaloric diet reduces UCP3 expression in individuals with obesity and the UCP3, PUN1 and PPARG2 expression correlate positively with carbohydrate oxidation and negatively with lipid oxidation. (C) 2017 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved. (AU)

• FAPESP's process: 13/08916-4 - Analysis of the expression of genes related to energy expenditure and lipid metabolism in individuals with grade III obesity before and after interventions for weight loss
• Grantee: Carla Barbosa Nonino
• Support Opportunities: Regular Research Grants