| Full text | |
| Author(s): |
Fratini, Paula
[1]
;
Rigoglio, Nathia Nathaly
[1]
;
Schiavo Matias, Gustavo de Sa
[1]
;
Carreira, Ana Claudia O.
[1, 2]
;
Rici, Rose Eli Grassi
[1]
;
Miglino, Maria Angelica
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, BR-05508270 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Biotechnol, Interunits Grad Program Biotechnol, Inst Biosci, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | BIORESEARCH OPEN ACCESS; v. 7, n. 1, p. 101-106, JUL 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
Regenerative medicine has been growing because of the emergent need for tissues/organs for transplants and restorative surgeries. Biological scaffolds are important tools to try to solve this problem. The one used in this reserach was developed by an acellular biological scaffold from canine placenta with a rich source of cellular matrix. After decellularization, the cellular matrix demonstrated structural preservation with the presence of important functional proteins such as collagen, fibronectin, and laminin. We used cells transduced with vascular endothelial growth factor (VEGF) to recellularize this scaffold. It was succeeded by seeding the cells in nonadherent plaques in the presence of the sterelized placenta scaffold. Cells were adhered to the scaffold when analyzed by immunocytochemistry and scanning electron microscopy, both showing sprouting of yolk sac VEGF (YSVEGF) cells. This recellularized scaffold is a promissory biomaterial for repairing injured areas where neovascularization is required. (AU) | |
| FAPESP's process: | 15/50466-1 - Advancing our understanding of Sertoli cell proliferation: insights from selected animal models |
| Grantee: | Maria Angelica Miglino |
| Support Opportunities: | Regular Research Grants |