Supplementing in vitro embryo production media by NPPC and sildenafil affect the cytoplasmic lipid content and gene expression of bovine cumulus-oocyte complexes and embryos

Author(s)

Botigelli, Ramon Cesar ^{[1, 2]} ; Razza, Eduardo Montanari ^[2] ; Pioltine, Elisa Mariano ^[2] ; Fontes, Patricia Kubo ^[2] ; Lancellotti Schwarz, Katia Regina ^[1] ; Verde Leal, Claudia Lima ^[1] ; Gouveia Nogueira, Marcelo Fabio ^{[2, 3]}

Affiliation

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, BR-13635900 Pirassununga, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Biosci, Dept Pharmacol, BR-18618689 Botucatu, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Sci Humanities & Languages, Dept Biol Sci, BR-19806900 Assis, SP - Brazil

• Document type: Journal article
• Source: REPRODUCTIVE BIOLOGY; v. 18, n. 1, p. 66-75, MAR 2018.
• Web of Science Citations: 2

Abstract

In our study, we added natriuretic peptide type C (NPPC) and/or sildenafil during in vitro maturation (IVM) of bovine cumulus-oocyte complexes (COCs) followed by in vitro culture (IVC) of embryos with or without sildenafil. We evaluated the effects on the lipid content (LC) of oocytes and embryos and also verified the expression of 96 transcripts related to competence in matured COCs and 96 transcripts related to embryo quality in blastocysts. After IVM, LC was decreased in oocytes by NPPC while sildenafil did not affect LC in oocytes. The genes involved in lipid metabolism and lipid accumulation (DGAT1, PLIN2and PLIN3) were not affected in COCs after treatment during IVM, although the expression of PTX3 (a cumulus cells expansion biomarker) was increased and the hatched blastocyst rate was increased by NPPC during IVM. During IVM, sildenafil increased the mRNA relative abundance of HSF1 and PAF1 and decreased REST in blastocysts. The use of sildenafil in IVC increased the LC of blastocysts. The mRNA abundance in blastocysts produced during IVC with sildenafil was changed for ATF4, XBP1, DNMT3A, DNMT3B, COX2, and SOX2. Although NPPC reduced the LC of oocytes after IVM and upregulated markers for cumulus expansion, embryo production was not affected and the produced blastocysts were able to regain their LC after IVC. Finally, the use of sildenafil during IVC increased the cytoplasmic LC of embryos but did not affect embryo quality, as measured by analysis of 96 transcripts related to embryo quality. (AU)

• FAPESP's process: 12/23409-9 - Differential gene expression, ultrastructural analysis and evaluation of the lipid profile of in vitro produced bovine blastocysts from oocytes matured conventionally or via SPOM system
• Grantee: Eduardo Montanari Razza
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/20379-0 - Studies regarding melatonin on the function and quality of bovine cumulus-oocyte complexes
• Grantee: Cláudia Lima Verde Leal
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/05083-1 - Modulation of oocyte nuclear maturation and its effects on cumulus-oocyte complexes and bovine embryo production in vitro
• Grantee: Marcelo Fábio Gouveia Nogueira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle
• Grantee: Marcelo Fábio Gouveia Nogueira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/07730-4 - Use of NPPC, folicular fluid and BMP15 to modulate oocyte meiotic arrest on in vitro maturation: effects on cumulus-oocyte complexes and bovine embryo production
• Grantee: Elisa Mariano Pioltine
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/00170-0 - The role of PDE5/cGMP on lipid metabolism in bovine oocytes
• Grantee: Kátia Regina Lancellotti Schwarz
• Support Opportunities: Scholarships in Brazil - Post-Doctoral