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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular and serological detection of Leishmania spp. in horses from an endemic area for canine visceral leishmaniasis in southeastern Brazil

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Author(s):
Benassi, Julia C. [1] ; Benvenga, Graziella U. [2] ; Ferreira, Helena L. [1, 2] ; Soares, Rodrigo M. [2] ; Silva, Diogo T. [2] ; Pereira, Vanessa F. [2] ; Ruiz, Vera Letticie A. [1] ; Oliveira, Tricia Maria F. de S. [1, 2]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Med Vet, Ave Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Programa Posgrad Epidemiol Expt Aplicada Zoonoses, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Pesquisa Veterinária Brasileira; v. 38, n. 6, p. 1058-1063, JUN 2018.
Web of Science Citations: 2
Abstract

This study aimed to verify the occurrence of Leishmania spp. and Leishmania (Leishmania) infantum in horses from a visceral leishmaniasis endemic area in Brazil. DNA samples from blood and conjunctival swab (CS) were tested by PCR and Indirect Immunofluorescence Antibody Test (IFAT). Although none of the horses was clinically sick, animals infected by Leishmania spp. were found and some could be characterized as infected by L. (L.) infantum. From 40 horses, 100% of the animals were positive by blood PCR, 90% (36/40) by CS PCR, and 2.5% (01/40) in serodiagnosis, by IFAT. Six from these 40 horses were L. (L.) infantum positive by blood PCR. Direct sequencing and analysis of amplicons resulted in a sequence to evolutionary analysis. Results indicate the presence of Leishmania spp. and L. (L.) infantum infecting healthy horses in Brazil. The presence of Leishmania spp. and L. (L.) infantum DNA in asymptomatic horses suggests that they can be important reservoirs of these parasites, a highly relevant finding for the epidemiological surveillance of the diseases they cause. (AU)

FAPESP's process: 11/00147-6 - Conjunctival swab samples for canine leishmaniasis detection by PCR
Grantee:Trícia Maria Ferreira de Sousa Oliveira
Support type: Regular Research Grants
FAPESP's process: 13/19821-4 - Conjunctival swab to Real Time PCR detection of canine visceral leishmaniasis in epidemiological surveys
Grantee:Trícia Maria Ferreira de Sousa Oliveira
Support type: Regular Research Grants