Electrochemical biosensor made with tyrosinase immobilized in a matrix of nanodiamonds and potato starch for detecting phenolic compounds

Author(s)

Camargo, Essica Rocha ^[1] ; Baccarin, Marina ^[2] ; Raymundo-Pereira, Paulo A. ^[3] ; Campos, Anderson M. ^[4] ; Oliveira, Geiser G. ^[2] ; Fatibello-Filho, Orlando ^[2] ; Oliveira, Jr., Osvaldo N. ^[3] ; Janegitz, Bruno C. ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Nat Sci Math & Educ, BR-13600970 Araras, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Chem, BR-13565970 Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil

^[4] Univ Sao Paulo, Sao Carlos Inst Chem, BR-13566590 Sao Carlos - Brazil

• Document type: Journal article
• Source: Analytica Chimica Acta; v. 1034, p. 137-143, NOV 30 2018.
• Web of Science Citations: 11

Abstract

The envisaged ubiquitous sensing and biosensing for varied applications has motivated materials development toward low cost, biocompatible platforms. In this paper, we demonstrate that carbon nanodiamonds (NDs) can be combined with potato starch (PS) and be deposited on a glassy carbon electrode (GCE) in the form of a homogeneous, rough film, with electroanalytical performance tuned by varying the relative ND-PS concentration. As a proof of concept, the ND/PS film served as matrix to immobilize tyrosinase (Tyr) and the resulting Tyr-ND-PS/GCE biosensor was suitable to detect catechol using differential pulse voltammetry with detection limit of 3.9 x 10(-7) mol L-1 in the range between 5.0 x 10(-6) and 7.4 x 10(-4) mol L-1. Catechol could also be detected in river and tap water samples. This high sensitivity, competitive with biosensors made with more sophisticated procedures and materials in the literature, is attributed to the large surface area and conductivity imparted by the small NDs (<5 nm). In addition, the ND-PS matrix may have its use extended to immobilize other enzymes and biomolecules, thus representing a potential biocompatible platform for ubiquitous biosensing. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 16/01919-6 - Design and fabrication of nanostructured flexible devices for biomarkers detection
• Grantee: Paulo Augusto Raymundo Pereira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/19099-2 - Development of electrochemical sensors and biosensors for different analytical purposes
• Grantee: Bruno Campos Janegitz
• Support Opportunities: Regular Research Grants