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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antimicrobial susceptibility patterns of Escherichia coli phylogenetic groups isolated from bovine clinical mastitis

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Author(s):
Tomazi, T. [1] ; Coura, F. M. [2] ; Goncalves, J. L. [1] ; Heinemann, M. B. [3] ; Santos, M. V. [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Nutr & Prod, BR-13635900 Pirassununga, SP - Brazil
[2] Fed Inst Minas Gerais, Dept Agr Sci, Campus Bambui, Rodovia Bambui Medeiros, Km 05, BR-38900000 Bambui, MG - Brazil
[3] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Prevent Vet Med & Anim Hlth, BR-05508900 Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF DAIRY SCIENCE; v. 101, n. 10, p. 9406-9418, OCT 2018.
Web of Science Citations: 1
Abstract

Determination of antimicrobial susceptibility (AMS) of Escherichia coli causing clinical mastitis (CM) according to the phylogenetic groups and its association with descriptors at the cow and herd level may help improve specific strategies for treatment and control of this pathogen in dairy herds. The aims of the present study were to (a) determine the frequency of phylogenetic groups of E. coli isolated from CM in dairy cows, and its association with cow-level descriptors (parity, lactation stage, CM severity, and affected quarter position), housing system, and season; and (b) determine and compare AMS among E. coli phylogenetic groups. A quadruplex PCR method was used to classify E. coli isolates into 1 of the 7 phylogenetic groups. Minimal inhibitory concentrations were determined for 10 antimicrobials, and survival analysis was performed to evaluate the AMS differences among E. coli phylogroups. Most E. coli isolates belonged to phylogroups A (52%) and B1 (38%). None of the cow- and herd-level descriptors were associated with the E. coli phylogenetic groups. Overall, E. coli isolates were mostly susceptible to ceftiofur (96.8%), sulfadimethoxine (75.5%), and cephalothin (74.5%). Based on the survival analysis, differences in AMS between phylogenetic groups of E. coli was observed only for cephalothin, in which strains of phylogroup A were inhibited at lower minimum inhibitory concentration than strains of phylogroup B1. Results of this study indicated low susceptibility of E. coli isolates identified from CM to most antimicrobials. In addition, differences in AMS can occur among E. coli phylogenetic groups, although they may be uncommon as they were limited to only one antimicrobial (i.e., cephalothin). (AU)

FAPESP's process: 13/22286-3 - Profile of etiologic agents causing clinical mastitis and antimicrobial use in dairy herds
Grantee:Tiago Tomazi
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/17411-6 - Economic impact and profile of etiologic agents of bovine mastitis
Grantee:Marcos Veiga dos Santos
Support Opportunities: Regular Research Grants
FAPESP's process: 15/10332-6 - Study of the molecular diversity of strains of Staphylococcus aureus isolated from bovine mastitis
Grantee:Marcos Bryan Heinemann
Support Opportunities: Regular Research Grants