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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antimicrobial and cytotoxic effects of denture base acrylic resin impregnated with cleaning agents after long-term immersion

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Author(s):
Procopio, A. L. F. [1] ; da Silva, R. A. [1] ; Maciel, J. G. [1] ; Sugio, C. Y. C. [1] ; Soares, S. [1] ; Urban, V. M. [2] ; Neppelenbroek, K. H. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Bauru Sch Dent, Al Octavio Pinheiro Brisola 9-75, BR-17012901 Bauru, SP - Brazil
[2] Univ Estadual Ponta Grossa, Dept Dent, Ave Gen Carlos Cavalcanti 4748, BR-84030900 Ponta Grossa, PR - Brazil
Total Affiliations: 2
Document type: Journal article
Source: TOXICOLOGY IN VITRO; v. 52, p. 8-13, OCT 2018.
Web of Science Citations: 1
Abstract

The coadjutant method for denture cleansing most used by denture wearers is immersion in chemical agents, which are toxic when in direct contact with cells. However, clinically, the contact between these chemical agents and prosthetic tissues does not occur directly, but rather with what remained impregnated into acrylic bases, even after rinsing the disinfected dentures. This study evaluated the antimicrobial and cytotoxic effects of a denture acrylic resin after successive cycles of daily overnight immersion in 1% sodium hypochlorite (1%NaClO) and 2% chlorhexidine digluconate (2%CHX), simulating the periods of 9 months or 1.5 year. Microbiological and cytotoxic assays were performed, respectively, by broth microdilution method (Candida albicans or Staphylococcus aureus) and MTT assay. Chemical residues of 2%CHX impregnated into the denture acrylic resin had an antimicrobial effect on both immersion periods, which was not observed with those of 1%NaClO. However, residues of 2%CHX were severely cytotoxic to human gingival fibroblasts compared to those of 1% NaClO and acrylic resin (not submitted to the denture cleansers), which were slightly cytotoxic. Even at low concentrations recommended for overnight soaking of removable dentures, the chemical residues of CHX may result in some degree of toxicity to the denture-bearing mucosa after long-term daily immersion. (AU)

FAPESP's process: 12/11074-2 - Modification of temporary resilient liners for dentures with antimicrobial agents: in vitro and in vivo studies
Grantee:Karin Hermana Neppelenbroek
Support Opportunities: Regular Research Grants