BGP-15 improves contractile function of regenerating soleus muscle

Author(s)

Nascimento, Tabata L. ^[1] ; Silva, Meiricris T. ^[1] ; Miyabara, Elen H. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Av Prof Lineu Prestes 2415, BR-05508000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY; v. 39, n. 1-2, p. 25-34, APR 2018.
• Web of Science Citations: 1

Abstract

This study investigated the effect of the heat shock protein inducer O-{[}3-piperidino-2-hydroxy-1-propyl]-nicotinic amidoxime (BGP-15) on the morphology and contractile function of regenerating soleus muscles from mice. Cryolesioned soleus muscles from young mice treated daily with BGP-15 (15mg/Kg) were evaluated on post-cryolesion day 10. At this time point, there was a significant decrease in the cross-sectional area of regenerating myofibers, maximal force, specific tetanic force, and fatigue resistance of regenerating soleus muscles. BGP-15 did not reverse the decrease in myofiber cross-sectional area but effectively prevented the reduction in tetanic force and fatigue resistance of regenerating muscles. In addition, BGP-15 treatment increased the expression of embryonic myosin heavy chain (e-MyHC), MyHC-II and MyHC-I in regenerating muscles. Although BGP-15 did not alter voltage dependent anion-selective channel 2 (VDAC2) expression in cryolesioned muscles, it was able to increase inducible 70-kDaheat shock protein(HSP70) expression. Our results suggest that BGP-15 improves strength recovery in regenerating soleus muscles by accelerating the re-expression of adult MyHC-II and MyHC-I isoforms and HSP70 induction. The beneficial effects of BGP-15 on the contractile function of regenerating muscles reinforce the potential of this molecule to be used as a therapeutic agent. (AU)

• FAPESP's process: 14/13874-1 - Skeletal muscle regeneration in mice with Marfan Syndrome: study of the regenerative potential of satellite cells
• Grantee: Meiricris Tomaz da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/23391-8 - Regenerative response of skeletal muscles from mice with Marfan Syndrome
• Grantee: Elen Haruka Miyabara
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/04783-0 - The influence of heat shock proteins in the recovery of skeletal muscle of old mice
• Grantee: Tábata Leal Nascimento
• Support Opportunities: Scholarships in Brazil - Doctorate