The P2X7 Receptor, Cathepsin S and Fractalkine in the Trigeminal Subnucleus Caudalis Signal Persistent Hypernociception in Temporomandibular Rat Joints

Author(s)

Bonfante, Ricardo ^[1] ; Napimoga, Marcelo Henrique ^[2] ; Macedo, Cristina Gomes ^[2] ; Adballa, Henrique Ballassini ^[1] ; Pieroni, Victor ^[2] ; Clemente-Napimoga, Juliana Trindade ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, UNICAMP, Lab Orofacial Pain, Dept Physiol Sci, Piracicaba Dent Sch, Piracicaba, SP - Brazil

^[2] Sao Leopoldo Mand Inst & Res Ctr, Rua Jose Rocha Junqueira 13, BR-13045755 Campinas, SP - Brazil

• Document type: Journal article
• Source: Neuroscience; v. 391, p. 120-130, NOV 1 2018.
• Web of Science Citations: 4

Abstract

Temporomandibular joint (TMJ) is frequently involved with rheumatoid arthritis with a high prevalence that could result in a chronic pain state. Once the disease is established in the joint, the antigen-specific immune reaction initiates a neuro-immune cascade of events that causes sensitization of the central nervous system. This study establishes animal experimental models that evaluate the chronicity of albumin-induced arthritis hypernociception in the TMJ. Antigen-induced arthritis was generated in rats with methylated bovine serum albumin (mBSA) diluted in complete Freund's. Intra-articular injection of mBSA (10 mu g/TMJ/week) during 3 weeks resulted in a persistent inflammatory hypernociception which was characterized by an inflammatory episode characterized by the increased of lymphocytes, macrophages and pro-inflammatory interleukins IL-12 and IL-18. The persistent model of inflammatory hypernociception induced by arthritis in the TMJ elicited protein levels of P2X7 receptors, cathepsin S and fractalkine in the trigeminal subnucleus caudalis. Overall, the results of the present work suggest that a persistent inflammatory hypernociception of albumin-induced arthritis in the TMJ leads to the activation of the central nervous system signaling by P2X7/cathepsin S/firactalkine pathway. (C) 2018 IBRO. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 16/16529-9 - Involvement of T cells and astrocytes of subnucleus caudalis in the inflammatory hipernociception induced by arthritis in the temporomandibular joint of rats.
• Grantee: Juliana Trindade Clemente Napimoga
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/09975-7 - Evaluation of microglial cells involvement in induction and persistence of inflammatory hypernociception induced by rheumatoid arthritis in rats TMJ
• Grantee: Ricardo Bonfante
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/00683-5 - Evaluation of the peripheral effect of 15d-PGJ2 on rheumatoid arthritis-induced inflammatory response in the TMJ of rats
• Grantee: Juliana Trindade Clemente Napimoga
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/18951-6 - Evaluation of inflammatory hyperalgesia in the progression of Rheumatoid Arthritis experimentally induced on rats temporomandibular joint
• Grantee: Ricardo Bonfante
• Support Opportunities: Scholarships in Brazil - Scientific Initiation