Isolation of spilanthol from Acmella oleracea based on Green Chemistry and evaluation of its in vitro anti-inflammatory activity

Author(s)

de Freitas Blanco, Veronica Santana ^{[1, 2]} ; Michalak, Barbara ^[3] ; Maccari Zelioli, Icaro Augusto ^[4] ; Santos de Oliveira, Adriana da Silva ^[5] ; Nova Rodrigues, Marili Villa ^[5] ; Ferreira, Antonio Gilberto ^[6] ; Garcia, Vera Lucia ^[5] ; Cabral, Fernando Antonio ^[4] ; Kiss, Anna Karolina ^[3] ; Ferreira Rodrigues, Rodney Alexandre ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Pharmacol Anesthesiol & Therapeut, Piracicaba, SP - Brazil

^[2] Univ Estadual Campinas, Dept Chem Nat Prod, Chem Biol & Agr Pluridisciplinary Res Ctr CPQBA, Campinas, SP - Brazil

^[3] Med Univ Warsaw, Dept Pharmacognosy & Mol Basis Phytotherapy, Warsaw - Poland

^[4] Univ Estadual Campinas, Dept Food Engn, Food Engn Fac, Lab Extract Appl Thermodynam & Equilibrium, Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Dept Organ & Pharmaceut Chem, Chem Biol & Agr Pluridisciplinary Res Ctr CPQBA, Campinas, SP - Brazil

^[6] Univ Fed Sao Carlos, Chem Dept, NMR Lab, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF SUPERCRITICAL FLUIDS; v. 140, p. 372-379, OCT 2018.
• Web of Science Citations: 1

Abstract

Acmella oleracea (jambu) is a South American plant whose pharmacological properties include antimalarial, anesthetic, and anti-inflammatory activities. However, the compounds contributing to the anti-inflammatory effect have received little attention. In this study, the active compound spilanthol was isolated using a Green Chemistry approach and presented significant anti-inflammatory activity in a lipopolysaccharide-activated human neutrophil model. First, the aerial parts of jambu were extracted and fractionated using supercritical carbon dioxide. The spilanthol-enriched fractions obtained were then subjected to further purification by flash chromatography, yielding spilanthol with 97% purity. Use of enzyme-linked immunosorbent assay (ELISA) revealed a reduction in the release of Interleukin-8 and Tumor Necrosis Factor - alpha by leukocytes exposed to spilanthol. In conclusion, this new approach enabled spilanthol to be obtained at high purity, in a fairly rapid procedure, while the results of the in vitro anti-inflammatory activity study indicated that the compound could be a promising new therapeutic agent. (AU)

• FAPESP's process: 14/06461-2 - Extraction of espilantol in the context of green chemistry and its application in the treatment of oral mucositis
• Grantee: Verônica Santana de Freitas Blanco
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/09644-3 - Non-isothermal separation process using supercritical technology and thermal diffusion
• Grantee: Fernando Antonio Cabral
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/07225-0 - Obtainment of N-alkilamide (Spilanthol) from aerial parts of jambu, using supercritical technology
• Grantee: Ícaro Augusto Maccari Zelioli
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 14/16186-9 - Development and evaluation of topical formulations for oral mucositis treatment containing Spilanthol
• Grantee: Rodney Alexandre Ferreira Rodrigues
• Support Opportunities: Regular Research Grants