Modulatory Effect of Polyphenolic Compounds from the Mangrove Tree Rhizophora mangle L. on Non-Alcoholic Fatty Liver Disease and Insulin Resistance in High-Fat Diet Obese Mice

Author(s):Show less -

de Souza Mesquita, Leonardo Mendes ^[1] ; Rabelo e Paiva Caria, Cintia ^[2] ; Santos, Paola Souza ^[2] ; Ruy, Caio Cesar ^[2] ; Lima, Natalia da Silva ^[2] ; Taketa Moreira, Debora Kono ^[2] ; da Rocha, Claudia Quintino ^{[1, 3]} ; Murador, Daniella Carisa ^[4] ; de Rosso, Veridiana Vera ^[4] ; Gambero, Alessandra ^[2] ; Vilegas, Wagner ^[1]

Affiliation

^[1] UNESP Sao Paulo State Univ, LBPN, Coastal Campus Sao Vicente, BR-11330900 Sao Vicente, SP - Brazil

^[2] USF Sao Francisco Univ, Clin Pharmacol & Gastroenterol Unit, Ave Sao Francisco de Assis 218, BR-12916900 Braganca Paulista, SP - Brazil

^[3] UFMA Fed Univ Maranhao, LEAF, Ave Portugueses, 1966 Bacanga, BR-65080805 Sao Luis, Maranhao - Brazil

^[4] Fed Univ Sao Paulo UNIFESP, Dept Biosci, Silva Jardim St 136, BR-11015020 Santos City, SP - Brazil

• Document type: Journal article
• Source: Molecules; v. 23, n. 9 SEP 2018.
• Web of Science Citations: 4

Abstract

No scientific report proves the action of the phytochemicals from the mangrove tree Rhizophora mangle in the treatment of diabetes. The aim of this work is to evaluate the effects of the acetonic extract of R. mangle barks (AERM) on type 2 diabetes. The main chemical constituents of the extract were analyzed by high-performance liquid chromatography (HPLC) and flow injection analysis electrospray-iontrap mass spectrometry (FIA-ESI-IT-MS/MS). High-fat diet (HFD)-fed mice were used as model of type 2 diabetes associated with obesity. After 4 weeks of AERM 5 or 50 mg/kg/day orally, glucose homeostasis was evaluated by insulin tolerance test (kiTT). Hepatic steatosis, triglycerides and gene expression were also evaluated. AERM consists of catechin, quercetin and chlorogenic acids derivatives. These metabolites have nutritional importance, obese mice treated with AERM (50 mg/kg) presented improvements in insulin resistance resulting in hepatic steatosis reductions associated with a strong inhibition of hepatic mRNA levels of CD36. The beneficial effects of AERM in an obesity model could be associated with its inhibitory alpha-amylase activity detected in vitro. Rhizophora mangle partially reverses insulin resistance and hepatic steatosis associated with obesity, supporting previous claims in traditional knowledge. (AU)

• FAPESP's process: 14/23951-3 - Standardized phytotherapics for the treatment of chronicle diseases: Rhizophora mangle
• Grantee: Leonardo Mendes de Souza Mesquita
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 09/52237-9 - Standardized herbal medicines for the treatment of chronic diseases
• Grantee: Wagner Vilegas
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants