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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Locus Coeruleus as a vigilance centre for active inspiration and expiration in rats

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Author(s):
Magalhaes, Karolyne S. [1] ; Spiller, Pedro F. [1] ; da Silva, Melina P. [1] ; Kuntze, Luciana B. [1] ; Paton, Julian F. R. [2, 3] ; Machado, Benedito H. [1] ; Moraes, Davi J. A. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Physiol, Ribeirao Preto, SP - Brazil
[2] Univ Bristol, Sch Physiol Pharmacol & Neurosci, Biomed Sci, Bristol, Avon - England
[3] Univ Auckland, Fac Med & Hlth Sci, Dept Physiol, Cardiovasc Auton Res Cluster, Auckland - New Zealand
Total Affiliations: 3
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 8, OCT 23 2018.
Web of Science Citations: 6
Abstract

At rest, inspiration is an active process while expiration is passive. However, high chemical drive (hypercapnia or hypoxia) activates central and peripheral chemoreceptors triggering reflex increases in inspiration and active expiration. The Locus Coeruleus contains noradrenergic neurons (A6 neurons) that increase their firing frequency when exposed to hypercapnia and hypoxia. Using recently developed neuronal hyperpolarising technology in conscious rats, we tested the hypothesis that A6 neurons are a part of a vigilance centre for controlling breathing under high chemical drive and that this includes recruitment of active inspiration and expiration in readiness for flight or fight. Pharmacogenetic inhibition of A6 neurons was without effect on resting and on peripheral chemoreceptors-evoked inspiratory, expiratory and ventilatory responses. On the other hand, the number of sighs evoked by systemic hypoxia was reduced. In the absence of peripheral chemoreceptors, inhibition of A6 neurons during hypercapnia did not affect sighing, but reduced both the magnitude and incidence of active expiration, and the frequency and amplitude of inspiration. These changes reduced pulmonary ventilation. Our data indicated that A6 neurons exert a CO2-dependent modulation of expiratory drive. The data also demonstrate that A6 neurons contribute to the CO2-evoked increases in the inspiratory motor output and hypoxia-evoked sighing. (AU)

FAPESP's process: 13/10484-5 - Electrophysiological and molecular characterization of neurons involved in the generation of respiratory rhythm and pattern of rats during postnatal development
Grantee:Davi José de Almeida Moraes
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 15/01073-7 - Electrophysiological characterization of expiratory motoneurons in rats submitted to sustained hypoxia
Grantee:Melina Pires da Silva
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/09071-9 - Participation of the noradrenergic neurons of locus coeruleus in the central generation of inspiratory and expiratory activities in response to the activation of the central chemoreceptors of rats
Grantee:Karolyne Silva Magalhães
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 16/24869-4 - Participation of lactate and carotid bodies in the respiratory responses of rats submitted to anaerobic physical exercise
Grantee:Pedro Favoretto Spiller
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/06077-5 - Changes in the neural networks involved with the generation and control of sympathetic and respiratory activities in different experimental models of hypoxia
Grantee:Benedito Honorio Machado
Support Opportunities: Research Projects - Thematic Grants