Abstract
The aim of our study is to investigate the medial prefrontal cortex (MPFC), in modulation of chronic pain through possible activation of descendent pathway during the neuropathic pain induced by chronic constriction of the sciatic nerve (CCSN). The role of MPFC and endogenous pain modulatory systems like periaqueductal gray matter (PAG), magnum raphe nucleus (MRN) and locus coeruleus (LC) will evaluate after stimulation of CCSN. Immunohistochemistry will perform at 7, 14, 21 and 28 days after stimulation of CCSN to identify c-Fos protein and NMDA glutamate, endocannabinoid CB1 and TRPV1 receptors, analyzing the neuromolecular activity. In addition, 28 days after the CCSN, the MPFC, PAG, MRN and LC will be separate and Western Blotting will perform to evaluate if chronic pain increases the expression of NMDA, CB1 and TRPV1 receptors. Furthermore, to verify the possible increase of amount of protein to these receptors is also accompanied by increase of mRNA in MPFC, SCP, NMR and LC will be used the technique of real-time polymerase chain reaction (RT-PCR). The next step will be the neurostimulation of MPFC through of technique of deep brain stimulation (DBS) evaluating its effect on the neuropathic pain, and also record the activity of neuronal nuclei of endogenous pain inhibitory system. The nociceptive test will be used is mechanical allodynia, the von Frey at 7, 14, 21 and 28 days after stimulation of CCSN. After evaluate the involvement of the activation of the cortical and the activation of NMDA, CB1 e TRPV1 receptors in the NP, the pharmacological interaction between the endocannabinoid and the glutamatergic and endocannabinoid and endovaniloid systems of MPFC will investigate. Furthermore, the comorbidity depression, anxiety and panic in rodents with chronic pain will investigate. The neurophysiological (neurostimulation by DBS) and neuropharmacological procedures (pharmacological interactions) will be also investigate. The animals will subject to experimental model of depression and anxiety/panic 21 days after the CCSN to evaluate the relation between depression or anxiety/panic disorders and neuropathic pain in Wistar rat. (AU)
Scientific publications
(11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MEDEIROS, PRISCILA;
OLIVEIRA-SILVA, MARIANA;
NEGRINI-FERRARI, SYLMARA ESTHER;
MEDEIROS, ANA CAROLINA;
ELIAS-FILHO, DAOUD HIBRAIM;
COIMBRA, NORBERTO CYSNE;
DE FREITAS, RENATO LEONARDO.
CB1-cannabinoid-, TRPV1-vanilloid- and NMDA-glutamatergic-receptor-signalling systems interact in the prelimbic cerebral cortex to control neuropathic pain symptoms.
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DEC 2020.
Web of Science Citations: 0.
MEDEIROS, PRISCILA;
NEGRINI-FERRARI, SYLMARA ESTHER;
PALAZZO, ENZA;
MAIONE, SABATINO;
FERREIRA, SERGIO HENRIQUE;
DE FREITAS, RENATO LEONARDO;
COIMBRA, NORBERTO CYSNE.
N-methyl-d-aspartate Receptors in the Prelimbic Cortex are Critical for the Maintenance of Neuropathic Pain.
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MEDEIROS, PRISCILA;
DE FREITAS, RENATO LEONARDO;
BOCCELLA, SERENA;
IANNOTTA, MONICA;
BELARDO, CARMELA;
MAZZITELLI, MARIACRISTINA;
ROMANO, ROSARIA;
DE GREGORIO, DANILO;
COIMBRA, NORBERTO CYSNE;
PALAZZO, ENZA;
MAIONE, SABATINO.
Characterization of the sensory, affective, cognitive, biochemical, and neuronal alterations in a modified chronic constriction injury model of neuropathic pain in mice.
Journal of Neuroscience Research,
AUG 2019.
Web of Science Citations: 0.
MATIAS JUNIOR, IVAIR;
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DE FREITA, RENATO LEONARDO;
VICENTE-CESAR, HILTON;
FERREIRA JUNIOR, JOSE RANIERY;
MACHADO, HELIO RUBENS;
MENEZES-REIS, RAFAEL.
Effective Parameters for Gait Analysis in Experimental Models for Evaluating Peripheral Nerve Injuries in Rats.
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DE FREITAS, RENATO LEONARDO;
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MEDEIROS, PRISCILA;
DA SILVA, JULIANA ALMEIDA;
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COIMBRA, NORBERTO CYSNE.
The endogenous opioid system modulates defensive behavior evoked by Crotalus durissus terrificus: Panicolytic-like effect of intracollicular non-selective opioid receptors blockade.
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Web of Science Citations: 4.
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HIBRAHIM ELIAS-FILHO, DAOUD;
FALCONI-SOBRINHO, LUIZ LUCIANO;
DE FREITAS, RENATO LEONARDO;
KHAN, ASMAT ULLAH;
DE OLIVEIRA, RICARDO;
COIMBRA, NORBERTO CYSNE.
Connexions between the dorsomedial division of the ventromedial hypothalamus and the dorsal periaqueductal grey matter are critical in the elaboration of hypothalamically mediated panic-like behaviour.
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DA SILVA, JULIANA ALMEIDA;
DE OLIVEIRA, RITHIELE CRISTINA;
DE OLIVEIRA, RICARDO;
ULLAH, FARHAD;
KHAN, ASMAT ULLAH;
COIMBRA, NORBERTO CYSNE.
THE mu(1)-OPIOID RECEPTOR AND 5-HT2A- AND 5HT(2C)-SEROTONERGIC RECEPTORS OF THE LOCUS COERULEUS ARE CRITICAL IN ELABORATING HYPOALGESIA INDUCED BY TONIC AND TONIC-CLONIC SEIZURES.
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