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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Application of an immobilized Rhizopus oryzae lipase to batch and continuous ester synthesis with a mixture of a lauric acid and fusel oil

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Author(s):
Boas, Renata N. Vilas [1] ; Ceron, Annie A. [1] ; Bento, Heitor B. S. [1] ; de Castro, Heizir F. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Engn Sch Lorena, BR-12602810 Lorena, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: BIOMASS & BIOENERGY; v. 119, p. 61-68, DEC 2018.
Web of Science Citations: 1
Abstract

Five commercial lipase preparations immobilized on epoxy-polysiloxane-hydroxyethylcellulose were tested as potential biocatalysts to mediate the esterification reaction of isoamyl alcohol with lauric acid. Among them, the immobilized lipase from Rhizopus oryzae was the most active biocatalyst, attained high ester yield (81.26%) in 24 h, being selected for subsequent tests using fusel oil as a source of isoamyl alcohol under batch and continuous runs. Under batch conditions, no adverse effect was found when pure isoamyl alcohol was replaced by fusel oil. However, under continuous runs, the selected biocatalyst was found to have limited performance (half-life time = 173 h) and the average ester productivity was only 277.8 +/- 19.3 mu mol g(-1) min(-1). These results can be credited to the high affinity of the hybrid matrix for the water formed as a by-product, modifying the substrate interaction with the essential water around the enzyme limiting the substrate migration to the solid phase and thereby reducing the enzyme activity. By replacing the hybrid support for a higher hydrophobic matrix such as commercial copolymer styrene-divinylbenzene it was possible to attain a stable operation, achieving an average ester productivity of 794.43 +/- 66.04 mu mol g(-1) min(-1) and increasing the half-life time to 285 h. Further productivity incremental (1598.68 +/- 135.79 mu mol g(-1) min(-1)) was also attained by using higher substrate loading. This process shows to be an attractive alternative to bulk chemical routes to satisfy increasing commercial demands for isoamyl laurate an essential emollient in the cosmetic industry. (AU)

FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants