Physicochemical, osteogenic and corrosion properties of bio-functionalized ZnO thin films: Potential material for biomedical applications

Author(s)

Trino, Luciana D. ^[1] ; Albano, Luiz G. S. ^[1] ; Bronze-Uhle, Erika S. ^[1] ; George, Anne ^[2] ; Mathew, Mathew T. ^[3] ; Lisboa-Filho, Paulo N. ^[1]

Affiliation

^[1] Sao Paulo State Univ Unesp, Sch Sci, BR-17033360 Bauru, SP - Brazil

^[2] Univ Illinois, Dept Oral Biol, Coll Dent, Chicago, IL 60612 - USA

^[3] Univ Illinois, Sch Med Rockford, Coll Med Rockford, Dept Biomed Sci, Rockford, IL 61107 - USA

• Document type: Journal article
• Source: CERAMICS INTERNATIONAL; v. 44, n. 17, p. 21004-21014, DEC 1 2018.
• Web of Science Citations: 1

Abstract

Nano-sized zinc oxide (ZnO) is well known for its antibacterial activity and biocompatibility, which make this material a promising candidate to tailor titanium (Ti) implant surfaces. In an optimized scenario, the antibacterial activity of ZnO and its biocompatibility can be envisioned as a good bio-functionalization strategy to increase osteointegration. Thus, in this work, it is proposed that the bio-functionalization of ZnO thin films with dentin matrix protein 1 (DMP1) peptides could function as an apatite crystal nucleator. Ti was coated with ZnO and functionalized with two different spacers, 3-(4-aminophenyl) propionic acid (APPA) or 3-mercaptopropionic acid (MPA) to facilitate binding with DMP1 peptides. Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) results confirmed the presence of the peptides on the ZnO thin film surface through characteristic bands related to amine and carboxylic acid groups and by the incidence of N 1 s spectra, respectively. Atomic force microscopy (AFM) images indicated that a more uniform layer of DMP1 peptides is formed in the the presence of the APPA and MPA spacers. In general, the results obtained showed that the bio-functionalized ZnO thin films with APPA spacer, ZnO APPA P sample, presented enhanced wettability (17 degrees), surface energy (72 dyn/cm), with an osteogenic surface and apatite nucleating properties. Furthermore, the electrochemical analysis showed increased corrosion resistance with noble E-OCP (-0.13 V), Ecorr (-0.46 V), and Icorr (8.91 x 10(-7) A/cm(2)) values. These findings indicated promising applications of ZnO APPA P in biomedical devices once it can accelerate the osteointegration process and improve the corrosion resistance of implants. (AU)

• FAPESP's process: 14/01713-3 - Functionalization and adsorption studies on thin oxide films
• Grantee: Luciana Daniele Trino
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/09963-6 - Construction and characterization of vertical organic field effect transistors by using nanostructured materials as intermediate electrode
• Grantee: Luíz Gustavo Simão Albano
• Support type: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
• Grantee: Elson Longo da Silva
• Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 14/27015-0 - Protein adhesion study in metal oxides thin films
• Grantee: Luciana Daniele Trino
• Support type: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 17/15035-5 - Adsorption of bisphosphonates and proteins on TiO2 surfaces for bone implants
• Grantee: Paulo Noronha Lisboa Filho
• Support type: Regular Research Grants