Fusel oil: Water adsorption and enzymatic synthesis of acetate esters in supercritical CO2

Author(s)

Baiao Dias, Arthur Luiz ^[1] ; da Cunha, Gabriela Nanuncio ^[1] ; dos Santos, Philipe ^[1] ; Meireles, M. Angela A. ^[1] ; Martinez, Julian ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Food Engn, Food Engn Dept, UNICAMP, BR-13083862 Campinas, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF SUPERCRITICAL FLUIDS; v. 142, p. 22-31, DEC 2018.
• Web of Science Citations: 1

Abstract

Acetate esters were produced from fusel oil through biocatalysed reactions under supercritical CO2 (SC-CO2) and Lipozyme 435 as catalyst. Low-pressure (P-atm/25 degrees C) and SC-CO2 adsorption with 13X-Na zeolites (15 MPa/40 degrees C) were applied to reduce the initial water of fusel oil. Pressure (10-20 MPa), temperature (40-60 degrees C) and enzyme to substrate molar ratio (3.12-12.50 g/mol OH) were investigated on the reaction conversion (Y, %). Infrared spectroscopy (FT-IR) analyses were carried out to identify chemical alterations. SC-CO2 reduced the initial water approximately 63% comparing to crude fusel oil. 10 MPa, 60 degrees C and 6.25 g/mol OH were the optimum conditions to produce acetate esters from crude oil. These conditions applied for adsorbed fusel oil increased the conversion of isoamyl, isobutyl and ethyl acetates up to 3-fold, 1.1-fold and 1.5-fold, respectively. FT-IR analyses suggested chemical changes in the bands of the reaction products. (AU)

• FAPESP's process: 15/11932-7 - Integration of high pressure extraction to separation operations for the recovery of by-products from fruit processing
• Grantee: Julian Martínez
• Support Opportunities: Regular Research Grants