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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Maternal immunization downregulates offspring TCD4 regulatory cells (Tregs) thymic maturation without implications for allergy inhibition

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de Oliveira, Marilia Garcia [1] ; de Lima Lira, Aline Aparecida [1] ; Sgnotto, Fabio da Ressureicao [2] ; Sabo Inoue, Amanda Harumi [1] ; Beltrame, Giovanna Rossi [1] ; da Silva, Debora [3] ; Menghini, Ricardo Palamar [3] ; da Silva Duarte, Alberto Jose [1, 4] ; Victor, Jefferson Russo [3, 1]
Total Authors: 9
[1] Univ Sao Paulo, Div Dermatol, Lab Med Invest LIM 56, Med Sch, Sao Paulo - Brazil
[2] Univ Sao Paulo, Div Hematol, Med Sch, Sao Paulo - Brazil
[3] Laureate Int Univ, Div Environm Hlth, FMU, Sao Paulo - Brazil
[4] Univ Sao Paulo, Div Pathol, Med Sch, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Scandinavian Journal of Immunology; v. 88, n. 6 DEC 2018.
Web of Science Citations: 1

The regulation of offspring allergy development mediated by maternal immunization was evidenced by several groups, and this mechanism seems to involve the induction of regulatory T cells (Tregs) on offspring. Here, we aimed to evaluate whether the effect of maternal immunization on offspring Tregs occurs as a result of peripheral or central modulation. Briefly, C57BL/6 female mice were immunized with OVA in Alum or Alum alone and boosted with OVA in saline or saline only after 10 and 20 days. Non-immunized offspring serum, thymus and spleen were evaluated at 3 or 20 days old, and some groups of pups were submitted to neonatal OVA-immunization protocol for the subsequent evaluation of antibody production and allergic response. Our experimental protocol could be validated because maternal OVA-immunization inhibited offspring allergic response as evidenced by the suppression of offspring IgE production and allergic lung inflammation. Interestingly, maternal immunization reduced the frequency of offspring thymic Tregs with an opposite effect on spleen Tregs. Furthermore, after neonatal immunization, the frequency of lung-infiltrated Tregs was also augmented on offspring from immunized mothers. In conclusion, maternal OVA-immunization can inhibit the thymic maturation of offspring Tregs without implications on peripheral Tregs induction and allergy inhibition. (AU)

FAPESP's process: 17/18558-9 - Influence of murine IgG repertoire on the maturation of B and gamma-deltaT cells with modulatory and/or regulatory potential in vitro
Grantee:Amanda Harumi Sabô Inoue
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 18/05181-7 - Translational evaluation of IgG effect upon the maturation of offspring thymus derived TCD4, TCD8, nTreg, gammadeltaT and e B cells.
Grantee:Alberto José da Silva Duarte
Support type: Regular Research Grants