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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Satellite DNAs Unveil Clues about the Ancestry and Composition of B Chromosomes in Three Grasshopper Species

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Author(s):
Milani, Diogo [1] ; Bardella, Vanessa B. [1] ; Ferretti, Ana B. S. M. [1] ; Palacios-Gimenez, Octavio M. [1, 2] ; Melo, Adriana de S. [3] ; Moura, Rita C. [3] ; Loreto, Vilma [4] ; Song, Hojun [5] ; Cabral-de-Mello, Diogo C. [1]
Total Authors: 9
Affiliation:
[1] UNESP Univ Estadual Paulista, Dept Biol, IB, BR-01049010 Rio Claro, SP - Brazil
[2] Uppsala Univ, Evolutionary Biol Ctr, Dept Evolutionary Biol, S-75236 Uppsala - Sweden
[3] UPE Univ Pernambuco, Inst Ciencias Biol, Lab Biodiversidade & Genet Insetos, BR-50100130 Recife, PE - Brazil
[4] UFPE Univ Fed Pernambuco, Dept Genet, CB, BR-50670901 Recife, PE - Brazil
[5] Texas A&M Univ, Dept Entomol, 2475 TAMU, College Stn, TX 77843 - USA
Total Affiliations: 5
Document type: Journal article
Source: GENES; v. 9, n. 11 NOV 2018.
Web of Science Citations: 3
Abstract

Supernumerary (B) chromosomes are dispensable genomic elements occurring frequently among grasshoppers. Most B chromosomes are enriched with repetitive DNAs, including satellite DNAs (satDNAs) that could be implicated in their evolution. Although studied in some species, the specific ancestry of B chromosomes is difficult to ascertain and it was determined in only a few examples. Here we used bioinformatics and cytogenetics to characterize the composition and putative ancestry of B chromosomes in three grasshopper species, Rhammatocerus brasiliensis, Schistocerca rubiginosa, and Xyleus discoideus angulatus. Using the RepeatExplorer pipeline we searched for the most abundant satDNAs in Illumina sequenced reads, and then we generated probes used in fluorescent in situ hybridization (FISH) to determine chromosomal position. We used this information to infer ancestry and the events that likely occurred at the origin of B chromosomes. We found twelve, nine, and eighteen satDNA families in the genomes of R. brasiliensis, S. rubiginosa, and X. d. angulatus, respectively. Some satDNAs revealed clustered organization on A and B chromosomes varying in number of sites and position along chromosomes. We did not find specific satDNA occurring in the B chromosome. The satDNAs shared among A and B chromosomes support the idea of putative intraspecific ancestry from small autosomes in the three species, i.e., pair S11 in R. brasiliensis, pair S9 in S. rubiginosa, and pair S10 in X. d. angulatus. The possibility of involvement of other chromosomal pairs in B chromosome origin is also hypothesized. Finally, we discussed particular aspects in composition, origin, and evolution of the B chromosome for each species. (AU)

FAPESP's process: 15/16661-1 - Sex and B chromosome enigmas: model systems for the study of chromosome and genome evolution
Grantee:César Martins
Support Opportunities: Research Projects - Thematic Grants