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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection

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Author(s):
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Moreno, Luisa Z. [1] ; Matajira, Carlos E. C. [1] ; Poor, Andre P. [1] ; Mesquita, Renan E. [1] ; Gomes, Vasco T. M. [1] ; Silva, Ana Paula S. [1] ; Amigo, Cristina R. [1] ; Christ, Ana Paula G. [2] ; Barbosa, Mikaela R. F. [2] ; Sato, Maria Ines Z. [2] ; Moreno, Andrea M. [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Sao Paulo - Brazil
[2] Companhia Ambiental Estado Sao Paulo CETESB, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: VETERINARY QUARTERLY; v. 38, n. 1, p. 1-8, DEC 7 2017.
Web of Science Citations: 2
Abstract

Background: Urinary tract infection (UTI) is a common disease in sows due to intensification of pig production. Despite direct economic losses, UTI prevalence and respective microbial identification are still poorly studied. Objective: The aims of this study were to identify the causative agents of UTI in sows through MALDI-TOF MS and to characterize their antimicrobial resistance profiles. Materials and Methods: Urine samples from 300 sows of three herds from Sao Paulo State (Brazil) were screened for UTI; suggestive samples were submitted to bacterial isolation. Species identification was performed by MALDI-TOF MS and susceptibility profiles were determined using disc diffusion method. Results: 128 samples suggestive of UTI were analyzed; 48% of the animals presented UTI caused by a single pathogen, while the remaining 52% presented mixed infection. Escherichia coli stood out with the highest frequency among both single and mixed infections. The Gram-positive were exclusively associated with 27% of single infections. The mixed infections were further classified into 49 profiles. The high frequency of multiresistant profiles stood out for most of the studied isolates. Conclusions: MALDI-TOF MS enabled the identification of rare pathogens related to UTI which may represent higher risk for porcine health, especially considering high frequency of multiresistant profiles. (AU)

FAPESP's process: 15/26159-1 - Comparative genome analysis of Streptococcus suis strains isolated from swine in Brazil
Grantee:Carlos Emilio Cabrera Matajira
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/25745-7 - Virulence assessment and phenotypic and genomic characterization of Corynebacterium spp. isolated from sow's urinary tract infection
Grantee:Luisa Zanolli Moreno
Support type: Scholarships in Brazil - Post-Doctorate