Bioactive glass-ceramic bone repair associated or not with autogenous bone: a study of organic bone matrix organization in a rabbit critical-sized calvarial model

Author(s)

Biguetti, Claudia Cristina ^{[1, 2]} ; Cavalla, Franco ^[3] ; Tim, Carla Roberta ^[4] ; Saraiva, Patricia Pinto ^[1] ; Orcini, Wilson ^[5] ; Holgado, Leandro De Andrade ^[6] ; Muniz Renno, Ana Claudia ^[7] ; Matsumoto, Mariza Akemi ^[8]

Affiliation

^[1] Univ Sagrado Coracao, Res & Postgrad Prorectory, Rua Irma Arminda 10-50, BR-17011160 Bauru, SP - Brazil

^[2] Univ Sao Paulo, FOB USP, Bauru Sch Dent, Oral Biol Doctorals Program, Alameda Octavio Pinheiro Brizola 9-75, BR-17012901 Bauru, SP - Brazil

^[3] Univ Chile, Sch Dent, Dept Conservat Dent, Sergio Livingstone 943, Santiago, RM - Chile

^[4] Univ Fed Sao Carlos, UFSCAR, Biotechnol Doctorals Program, Rodovia Washington Luis, Km 235, SP-310, BR-13565905 Sao Carlos, SP - Brazil

^[5] USC, Res & Postgrad Prorectory, Mol Biol Lab, Rua Irma Arminda 10-50, BR-17011160 Jardim - Brazil

^[6] USC, Dept Hlth Sci, Discipline Oral & Maxillofacial Surg, Rua Irma Arminda 10-50, BR-17011160 Jardim Brasil Bauru, SP - Brazil

^[7] Univ Fed Sao Paulo, Dept Biosci, Campus Baixada Santista UNIFESP, BR-11060001 Santos, SP - Brazil

^[8] Sao Paulo State Univ, FOA UNESP, Dept Basic Sci, Discipline Histol & Embriol, Rua Paul Harris 1100, Casa 03, BR-18016110 Jardim Nova Iorque Araca, SP - Brazil

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 23, n. 1, p. 413-421, JAN 2019.
• Web of Science Citations: 0

Abstract

ObjectiveThe aim of the study was to analyze bone matrix (BMX) organization after bone grafting and repair using a new bioactive glass-ceramic (Biosilicate((R))) associated or not with particulate autogenous bone graft.Material and methodsThirty rabbits underwent surgical bilateral parietal defects and divided into groups according to the materials used: (C) controlblood clot, (BG) particulate autogenous bone, (BS) bioactive glass-ceramic, and BG + BS. After 7, 14, and 30days post-surgery, a fragment of each specimen was fixed in -80 degrees C liquid nitrogen for zymographic evaluation, while the remaining was fixed in 10% formalin for histological birefringence analysis.ResultsThe results of this study demonstrated that matrix organization in experimental groups was significantly improved compared to C considering collagenous organization. Zymographic analysis revealed pro-MMP-2, pro-MMP-9, and active (a)-MMP-2 in all groups, showing gradual decrease of total gelatinolytic activity during the periods. At day 7, BG presented more prominent gelatinolytic activity for pro-MMP-2 and 9 and a-MMP-2, when compared to the other groups. In addition, at day 7, a 53% activation ratio (active form/{[}active form + latent form]) was evident in C group, 33% in BS group, and 31% in BG group.ConclusionIn general, BS allowed the production of a BMX similar to BG, with organized collagen deposition and MMP-2 and MMP-9 disponibility, permitting satisfactory bone remodeling at the late period.Clinical relevanceThe evaluation of new bone substitute, with favorable biological properties, opens the possibility for its use as a viable and efficient alternative to autologous bone graft. (AU)

• FAPESP's process: 09/17294-1 - Repair of rabbits calvaria bone defects using bioactive glass-ceramic: biosilicate
• Grantee: Gustavo Caviquioli
• Support Opportunities: Scholarships in Brazil - Scientific Initiation