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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

High Prevalence of Multidrug-Resistant Klebsiella pneumoniae Harboring Several Virulence and beta-Lactamase Encoding Genes in a Brazilian Intensive Care Unit

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Ferreira, Roumayne L. [1, 2] ; da Silva, Brenda C. M. [1] ; Rezende, Graziela S. [1] ; Nakamura-Silva, Rafael [3] ; Pitondo-Silva, Andre [3] ; Campanini, Emeline Boni [1] ; Brito, Marcia C. A. [2] ; da Silva, Eulalia M. L. [4] ; de Melo Freire, Caio Cesar [1] ; da Cunha, Anderson F. [1] ; da Silva Pranchevicius, Maria-Cristina [1]
Total Authors: 11
[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP - Brazil
[2] Lab Cent Saude Publ Tocantins, Palmas - Brazil
[3] Univ Ribeirao Preto, Sch Dent, Ribeirao Preto - Brazil
[4] Oklahoma Med Res Fdn, Dept Cell Cycle & Canc Biol, 825 NE 13th St, Oklahoma City, OK 73104 - USA
Total Affiliations: 4
Document type: Journal article
Web of Science Citations: 4

Klebsiella pneumoniae is an important opportunistic pathogen that commonly causes nosocomial infections and contributes to substantial morbidity and mortality. We sought to investigate the antibiotic resistance profile, pathogenic potential and the clonal relationships between K. pneumoniae (n = 25) isolated from patients and sources at a tertiary care hospital's intensive care units (ICUs) in the northern region of Brazil. Most of K. pneumoniae isolates (n = 21, 84%) were classified as multidrug resistant (MDR) with high-level resistance to beta-lactams, aminoglycosides, quinolones, tigecycline, and colistin. All the 25 isolates presented extended-spectrum beta-lactamase-producing (ESBL), including carbapenemase producers, and carried the bla(KPC) (100%), bla(TEM) (100%), bla(SHV) variants (n = 24, 96%), bia(OXA-1) group (n = 21, 84%) and bla(CTX\_M\_1) group (n = 18, 72%) genes. The K2 serotype was found in 4% (n = 1) of the isolates, and the K1 was not detected. The virulence-associated genes found among the 25 isolates were mrkD (n = 24, 96%), fimH-1 (n = 22, 88%), entB (100%), iutA (n = 10, 40%), ybtS (n = 15, 60%). The genes related with efflux pumps and outer membrane porins found were AcrAB (100%), to/C (n = 24, 96%), mdtK (n = 22, 88%), OmpK35 (n = 15, 60%), and OmpK36 (n = 7, 28%). ERIC-PCR was employed to determine the clonal relationship between the different isolated strains. The obtained ERIC-PCR patterns revealed that the similarity between isolates was above 70%. To determine the sequence types (STs) a multilocus sequence typing (MLST) assay was used. The results indicated the presence of high-risk international clones among the isolates. In our study, the wide variety of MDR K. pneumoniae harboring beta-lactams and virulence genes strongly suggest a necessity for the implementation of effective strategies to prevent and control the spread of antibiotic resistant infections. (AU)

FAPESP's process: 13/22581-5 - Study of pathogenic mechanisms and correlation with antimicrobial resistance of Klebsiella pneumoniae from Brazil and other countries across five continents
Grantee:André Pitondo da Silva
Support type: Research Grants - Young Investigators Grants