| Full text | |
| Author(s): |
Carla Rossini Crepaldi
[1]
;
Giovana Krempel Fonseca Merighe
[2]
;
Hélen Julie Laure
[3]
;
José César Rosa
[4]
;
Flávio Vieira Meirelles
[5]
;
Marcelo de Cerqueira César
[6]
Total Authors: 6
|
| Affiliation: | [1] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Ciências Básicas - Brasil
[2] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Ciências Básicas - Brasil
[3] USP. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos - Brasil
[4] USP. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos - Brasil
[5] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Ciências Básicas - Brasil
[6] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Ciências Básicas - Brasil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Pesquisa Veterinária Brasileira; v. 33, p. 45-50, 2013-12-00. |
| Abstract | |
Cell culture methods are used for studies of protein interactions in neural cells, helping to detect the interactome of proteins linked to generation of central nervous system diseases. For this reason, neural cells and neurospheres isolated from cortical chicken brain are a current model for studies of neurological diseases, such as epilepsy. Chicken brain has key characteristics on its proteome, with a differential expression of proteins linked to energy metabolism, some of them (VDAC 1 and VDAC 2) play an important role in understanding mechanism of refractory epilepsy. Using the methods described, we found neurospheres, in which cells grow in structures with the ideal diameter of 100-200µm within seven days after isolation. Neurospheres differentiation was obtained after adhesion of these cells to surfaces coated with poly-D-Lysine, detected by migration of fibers inside them. Unlike neurospheres, neurons extended neurites after 11 days of isolation. Here we describe a method to isolate and culture neurons and neurospheres from chicken cerebral cortex. Such "in vitro" model can be utilized on studies of neuronal protein differential expression and interaction. Cultures of isolated neurons represent an accessible model on studies of apoptosis and channel blockers of key proteins linked to brain metabolism. (AU) | |
| FAPESP's process: | 09/06687-2 - Proteomic study of apoptosis in avian neurons: the role of VDAC 1 and VDAC 2 |
| Grantee: | Carla Rossini Crepaldi |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 10/05560-6 - Interactomic analysis of hexokinase-VDAC-ANT complex in rat, bovine and chicken neuronal cells |
| Grantee: | Marcelo de Cerqueira César |
| Support Opportunities: | Regular Research Grants |