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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of sucralfate enema in experimental diversion colitis

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Author(s):
José Aires Pereira [1] ; Murilo Rocha Rodrigues [2] ; Daniela Tiemi Sato [3] ; Paulo Pedroso Silveira Júnior [4] ; Alice Moreira Dias [5] ; Camila Gonçalves da Silva [6] ; Carlos Augusto Real Martinez [7]
Total Authors: 7
Affiliation:
[1] Universidade São Francisco. School of Medicine - Brasil
[2] Universidade São Francisco. School of Medicine - Brasil
[3] Universidade São Francisco. School of Medicine - Brasil
[4] Universidade São Francisco. School of Medicine - Brasil
[5] Universidade São Francisco. School of Medicine - Brasil
[6] Universidade Estadual de Campinas. Biological Sciences Institute - Brasil
[7] Universidade São Francisco. Post-graduation Program in Health Sciences - Brasil
Total Affiliations: 7
Document type: Journal article
Source: J. Coloproctol. (Rio J.); v. 33, n. 4, p. 182-190, 2013-12-00.
Abstract

Diversion colitis (DC) is an inflammatory disease that develops in segments with fecal diversion. Sucralfate (SCF) complex, which consists of sucrose octasulfate and polyaluminum hydroxide, has been demonstrated to be effective in the treatment of different forms of colitis. However, until now, the effects of SCF have not been evaluated in DC. OBJECTIVE: to evaluate whether the use of enemas containing SFC improves histological findings in experimental DC. METHODS: Thirty-six rats underwent right colon bypass procedure through the creation of a proximal colostomy and a distal mucous fistula. The animals were divided into two groups according to the euthanization procedure to be performed two to four weeks after surgery. Each experimental group was divided into three subgroups of six animals, which were submitted to daily application of enemas containing saline solution 0.9% or SCF at concentrations of 1.0 g/kg/day or 2.0 g/kg/day, respectively. The diagnosis of DC in segments with fecal diversion was established by histopathological study considering the following variables: epithelial loss, formation of crypt abscesses, the population of goblet cells, inflammatory infiltrate and presence of fibrosis. For statistical analysis, the nonparametric Mann-Whitney and Kruskal-Wallis tests were used, with a significance level of 5% (p <0.05). RESULTS: It was observed that the daily application of SCF enemas decreased epithelial loss, formation of colon crypt abscesses, inflammatory infiltrate and tissue fibrosis (p <0.05), unrelated to time of intervention. The intervention with SCF preserves the goblet cell population. The effects of the substance on the preservation of colonic epithelium; the decrease in the inflammatory process and subsequent abscess formation in the colon crypts are associated with the concentration used, whereas tissue fibrosis decrease is associated with the concentration and time of intervention. CONCLUSION: Preventive application of SCF enemas reduces the inflammatory process in the colon with fecal diversion (AU)

FAPESP's process: 10/12492-7 - Evaluation of antioxidant effects of 5-aminosalycylic acid, N-acetylcysteine, sucralfate, curcumin and aqueous extract of Ilex paraguariensis in contente and pattern of tissue expression of intercellular junctions proteins: study in rats
Grantee:Carlos Augusto Real Martinez
Support Opportunities: Regular Research Grants