| Full text | |
| Author(s): |
Vanda Renata Reis
[1]
;
Ana Teresa Burlamaqui Faraco Antonangelo
[2]
;
Ana Paula Guarnieri Bassi
[3]
;
Débora Colombi
[4]
;
Sandra Regina Ceccato-Antonini
[5]
Total Authors: 5
|
| Affiliation: | [1] Universidade Federal de São Carlos. Centro de Ciências Agrarias. Departamento Tecnologia Agroindustrial e Socio-Economia Rural - Brasil
[2] Universidade Estadual Paulista. Instituto de Biociencias de Botucatu. Departamento Parasitologia - Brasil
[3] Universidade Federal de São Carlos. Centro de Ciências Agrarias. Departamento Tecnologia Agroindustrial e Socio-Economia Rural - Brasil
[4] Genotyping Laboratório de Biotecnologia Ltda - Brasil
[5] Universidade Federal de São Carlos. Centro de Ciências Agrarias. Departamento Tecnologia Agroindustrial e Socio-Economia Rural - Brasil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Brazilian Journal of Microbiology; v. 48, n. 2, p. 268-274, 2017-06-00. |
| Abstract | |
Abstract Strains of Saccharomyces cerevisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cerevisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cerevisiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The results obtained from the phenotypic tests by Principal-Component Analysis revealed that unlike the smooth colonies, the rough colonies of S. cerevisiae exhibit an enhanced resistance to stressful conditions resulting from the presence of excessive glucose and ethanol and high sedimentation rate. The microsatellite analysis was not successful to distinguish between the colony phenotypes as phenotypic assays. The relevant industrial strain PE-2 was observed in close genetic proximity to rough-colony although it does not display this colony morphology. A unique genetic pattern specific to a particular phenotype remains elusive. (AU) | |
| FAPESP's process: | 09/14617-4 - Morphological, physiological and genetic characterization of contaminant yeasts from the alcoholic fermentation and evaluation of their competitive potentiality based on the resistance to stress during fermentation |
| Grantee: | Sandra Regina Ceccato Antonini |
| Support Opportunities: | Regular Research Grants |