Identification of new bioactive peptides from Kefir milk through proteopeptidomics: Bioprospection of antihypertensive molecules

Author(s):Show less -

Amorim, Fernanda Gobbi ^[1] ; Coitinho, Luciana Barbosa ^[2] ; Dias, Ananda Tissianel ^[3] ; Freitas Friques, Andreia Gomes ^{[1, 3]} ; Monteiro, Brenna Lepaus ^[3] ; Dias de Rezende, Lucas Cunha ^[4] ; Costa Pereira, Thiago de Melo ^[1] ; Campagnaro, Bianca Prandi ^[1] ; De Pauw, Edwin ^[5] ; Vasquez, Elisardo Corral ^[1] ; Quinton, Loic ^[5]

Affiliation

^[1] Vila Velha Univ, Pharmaceut Sci Grad Program, Vila Velha, ES - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[3] Univ Fed Espirito Santo, Lab Translat Physiol, Vitoria, ES - Brazil

^[4] Univ Fed Espirito Santo, CEUNES, Dept Nat Sci, Sao Mateus, ES - Brazil

^[5] Univ Liege, Dept Chem, Lab Mass Spectrometry, Liege - Belgium

• Document type: Journal article
• Source: Food Chemistry; v. 282, p. 109-119, JUN 2019.
• Web of Science Citations: 7

Abstract

Kefir, a probiotic beverage prepared from fermented milk, has been associated with antihypertensive activity. However, the bioactive molecules responsible for this activity still remain unclear. Therefore, in this study we aim to evaluate the mechanisms of the antihypertensive effects of Kefir in the two-kidney one-clip hypertension model, and to bioprospect for bioactive peptides identified by proteomic methodologies. Treatment with Kefir was performed via gavage, and resulted in a 37 mmHg reduction in systolic arterial pressure and 19% inhibition of angiotensin converting enzyme (ACE) activity. For the proteopeptidomic study, the protein extract of Kefir beverage and non-fermented bovine milk were analysed by MALDI-TOF mass spectrometry, and their tryptic digestion products sequenced via Shotgun proteomics (Q-Exactive mass spectrometer). A list of 35 peptides with potential hypertensive activity due to ACE inhibition were identified. These results demonstrate the benefits of Kefir products, and may guide the design of new antihypertensive drugs. (AU)

• FAPESP's process: 14/01497-9 - Biotransformation of lapachol by filamentous fungi: proteomic analysis for identification to biotransformation route
• Grantee: Luciana Barbosa Coitinho
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/12516-0 - Analysis of secretome from Phanerochaete chrysosporium and post-translational modifications of some proteins during the lapachol biotransformation process by mass spectrometry
• Grantee: Luciana Barbosa Coitinho
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate