| Full text | |
| Author(s): |
Nava, Roseane Galdioli
[1]
;
Oliveira-Silva, Mariana
[2]
;
Nakamura-Silva, Rafael
[2]
;
Pitondo-Silva, Andre
[2]
;
Vespero, Eliana Carolina
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Estadual Londrina, Dept Pathol Clin & Toxicol Anal, Londrina, Parana - Brazil
[2] Univ Ribeirao Preto, Sch Dent, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES; v. 79, p. 101-103, FEB 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
Objectives: The aim of this study was to investigate the bla(NDM) gene, pathogenic potential, and antimicrobial resistance of clinical isolates of carbapenem-resistant Klebsiella pneumoniae isolated from patients admitted to the University Hospital of Londrina between January 2014 and March 2017. Methods: bla(NDM-1) and virulence genes were investigated using conventional PCR methods Antimicrobial susceptibility testing was performed by disk diffusion method according to the Clinical and Laboratory Standards Institute guidelines of 2017. Clonal relationships of the New Delhi metallo-beta-lactamase (NDM)-positive isolates were determined by enterobacterial repetitive intergenic consensus (ERIC)-PCR and multilocus sequence typing (MLST). Results: A total of 825 K. pneumoniae were identified, with four isolates (Kp6408, Kp6410, Kp6411, and Kp6715) presenting the bla(NDM-1) gene. All NDM-1-producing isolates showed co-production of bla(KPC-2) and bla(TEM) genes and also the virulence genes kfu, entB, mrkD, and fimH. Three isolates (Kp6408, Kp6410, and Kp6715) were classified as multidrug-resistant (MDR) and one (Kp6411) as extensively drugresistant (XDR). ERIC-PCR analyses demonstrated that the isolates shared about 60% genetic similarity. MLST revealed four different sequence types (STs), described for the first time in Brazil, with two novel STs described in this study: ST3371 and ST3372. Conclusion: This study reports the identification of NDM-1 associated with KPC and virulence genes in four MDR K. pneumoniae with STs first described in Brazil. (c) 2018 State University of Londrina. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). (AU) | |
| FAPESP's process: | 13/22581-5 - Study of pathogenic mechanisms and correlation with antimicrobial resistance of Klebsiella pneumoniae from Brazil and other countries across five continents |
| Grantee: | André Pitondo da Silva |
| Support Opportunities: | Research Grants - Young Investigators Grants |