Role of hypercholesterolemia in modifying the composition of lipid rafts microdoma...
| Full text | |
| Author(s): |
Tsubone, Tayana Mazin
[1]
;
Junqueira, Helena Couto
[2]
;
Baptista, Mauricio S.
[2]
;
Itri, Rosangela
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Inst Phys, Dept Appl Phys, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES; v. 1861, n. 3, p. 660-669, MAR 1 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Lipid rafts display a lateral heterogeneity forming membrane microdomains that hold a fundamental role on biological membranes and are indispensable to physiological functions of cells. Oxidative stress in cellular environments may cause lipid oxidation, changing membrane composition and organization, thus implying in effects in cell signaling and even loss of homeostasis. The individual contribution of oxidized lipid species to the formation or disruption of lipid rafts in membranes still remains unknown. Here, we investigate the role of different structures of oxidized phospholipids on rafts microdomains by carefully controlling the membrane composition. Our experimental approach based on fluorescence microscopy of giant unilamellar vesicles (GUV) enables the direct visualization of the impact of hydroperoxidized POPC lipid (referred to as POPCOOH) and shortened chain lipid PazePC (1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine) on phase separation. We found that the molecular structure of oxidized lipid is of paramount importance on lipid mixing and/or de mixing. The hydrophobic mismatch promoted by POPCOOH coupled to its cylindrical molecular shape favor microdomains formation. In contrast, the conical shape of PazePC causes disarrangement of lipid 2D organized platforms. Our findings contribute to better unraveling how oxidized phospholipids can trigger formation or disruption of lipid rafts. As a consequence, phospholipid oxidation may indirectly affect association or dissociation of key biomolecules in the rafts thus altering cell signaling and homeostasis. (AU) | |
| FAPESP's process: | 16/23071-9 - Photosensitization in lysosomal mimetic membranes to investigate the cell death mechanism related to autophagy |
| Grantee: | Tayana Mazin Tsubone |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 14/20107-7 - Lipid oxidation in membrane and cell biophysics: from functional nanosensors to impact on amyloid formation. Application of advanced fluorescence, X-ray scattering and microscopy techniques |
| Grantee: | Rosangela Itri |
| Support type: | Regular Research Grants |
| FAPESP's process: | 11/00963-8 - Photosensitization mechanisms in lipid membranes: the role of lipid hydroperoxides and the lipid rafts formation |
| Grantee: | Helena Couto Junqueira |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 12/50680-5 - Photosensitization in life sciences |
| Grantee: | Mauricio da Silva Baptista |
| Support type: | Research Projects - Thematic Grants |