Late effect of larval co-exposure to the insecticide clothianidin and fungicide pyraclostrobin in Africanized Apis mellifera

Author(s)

Tadei, Rafaela ^{[1, 2]} ; Domingues, Caio E. C. ^[3] ; Malaquias, Jose Bruno ^[4] ; Camilo, Erasnilson Vieira ^[5] ; Malaspina, Osmar ^[3] ; Silva-Zacarin, Elaine C. M. ^{[1, 2]}

Affiliation

^[1] Univ Fed Sao Carlos UFSCar, Programa Posgrad Biotecnol & Monitoramento Ambien, CCTS, Campus Sorocaba, BR-18052780 Sorocaba, SP - Brazil

^[2] Univ Fed Sao Carlos UFSCar, Dept Biol, CCHB, Campus Sorocaba, BR-18052780 Sorocaba, SP - Brazil

^[3] Univ Estadual Paulista UNESP Julio de Mesquita Fi, Programa Posgrad Biol Celular & Mol, Campus Rio Claro, Ave 24 A, 1515, BR-13506900 Rio Claro, SP - Brazil

^[4] Univ Sao Paulo, Luiz de Queiroz Coll Agr ESALQ, Dept Entomol & Acarol, Ave Padua Dias 11, BR-13418900 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Luiz de Queiroz Coll Agr ESALQ, Dept Agr Stat & Experimentat, Ave Padua Dias 11, BR-13418900 Sao Paulo - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 9, MAR 1 2019.
• Web of Science Citations: 4

Abstract

Among the factors that contribute to the reduction of honeybee populations are the pesticides. These chemical compounds reach the hive through forager bees, and once there, they can be ingested by the larvae. We evaluated the effects of repeated larval exposure to neonicotinoid insecticide, both in isolation and in combination with strobilurin fungicide, at environmentally relevant doses. The total consumption of the contaminated diet was 23.63 ng fungicide/larvae (pyraclostrobin) and 0.2364 ng insecticide/larvae (clothianidin). The effects on post-embryonic development were evaluated over time. Additionally, we assessed the survival pattern of worker bees after emergence, and the pesticides' effects on the behavior of newly emerged workers and young workers. Young bees that were exposed to the fungicide and those subjected to co-exposure to both pesticides during larval phase showed behavioral changes. The insecticide, both in isolation and in combination with fungicide reduced the bees' longevity; this effect of larval exposure to pesticides was stronger in bees that were exposed only to the insecticide. Although the larvae did not have sensitivity to exposure to pesticides, they showed later effects after emergence, which may compromise the dynamics of the colony, contributing to the reduction of the populations of bees in agroecosystems. (AU)

• FAPESP's process: 16/15743-7 - Analysis of effects of strobilurin fungicide pyraclostrobin in midgut of Africanized Apis mellifera Linnaeus, 1758 (Hymenoptera: Apidae) and Melipona scutellaris Latreille, 1811 (Hymenoptera: Apidae)
• Grantee: Caio Eduardo da Costa Domingues
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use
• Grantee: Osmar Malaspina
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 16/26043-6 - Toxicological evaluation of larval exposure to the mixture of the clothianidin neonicotinoid insecticide and the strobilurin pyraclostrobin fungicide on the development of Africanized Apis mellifera
• Grantee: Rafaela Tadei
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/20380-8 - Ecological modelling of the larval dispersion of Spodoptera frugiperda (Lepidoptera: Noctuidae) on Bt cotton: implications for evolution and resistance management
• Grantee: José Bruno Malaquias
• Support Opportunities: Scholarships in Brazil - Doctorate