Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil

Author(s):Show less -

Ribeiro, Geovani de Oliveira ^[1] ; Costa Monteiro, Fred Julio ^[2] ; Rego, Marlisson Octavio da S. ^[2] ; D'Athaide Ribeiro, Edcelha Soares ^[2] ; de Castro, Daniela Funayama ^[3] ; Caseiro, Marcos Montani ^[3] ; Souza Marinho, Robson dos Santos ^[4] ; Komninakis, Shirley Vasconcelos ^{[4, 5]} ; Witkin, Steven S. ^{[6, 7]} ; Deng, Xutao ^{[8, 9]} ; Delwart, Eric ^{[8, 9]} ; Sabino, Ester Cerdeira ^{[6, 10]} ; da Costa, Antonio Charlys ^[6] ; Leal, Elcio ^[1]

Affiliation

^[1] Fed Univ Para, Inst Biol Sci, Ave Augusto Correa01, BR-66075000 Belem, Para - Brazil

^[2] Superintendencia Vigilancia Saude Amapa, Lab Vetores, Rua Tancredo Neves 1-118, BR-68905230 Macapa, AP - Brazil

^[3] Lusiada Univ, Rua Oswaldo Cruz 179, BR-11045101 Santos, SP - Brazil

^[4] Univ Fed Sao Paulo, Lab Retrovirol, Rua Pedro de Toledo 781, BR-04039032 Sao Paulo, SP - Brazil

^[5] Fac Med ABC, BR-09060870 Santo Andre, SP - Brazil

^[6] Univ Sao Paulo, Inst Trop Med, Ave Dr Eneas Carvalho de Aguiar 470, BR-05403000 Sao Paulo - Brazil

^[7] Weill Cornell Med, Dept Obstet & Gynecol, 407 E 61st St, New York, NY 10065 - USA

^[8] Vitalant Res Inst, 270 Masonic Ave, San Francisco, CA 94118 - USA

^[9] Univ Calif San Francisco, Dept Lab Med, Blood Syst Res Inst, 270 Masonic Ave, San Francisco, CA 94118 - USA

^[10] Univ Sao Paulo, Dept Infect Dis, Fac Med, Ave Dr Arnaldo 455 Cerqueira Cesar, BR-01246903 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Viruses-Basel; v. 11, n. 2 FEB 2019.
• Web of Science Citations: 0

Abstract

Advancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. Hubei reo-like virus 7 (HRLV 7) was recently detected by the virome analysis of fecal samples from migratory birds in Australia. We now report the detection of RNA-dependent RNA polymerase sequences of HRLV 7 in pools of Aedes aegypti and Culex quinquefasciatus mosquitoes species from the Brazilian Amazon forest. Phylogenetic inferences indicated that all HRLV 7 strains fall within the same independent clade. In addition, HRLV 7 shared a close ancestral lineage with the Dinovernavirus genus of the Reoviridae family. Our findings indicate that HRLV 7 is present in two species of mosquitoes. (AU)

• FAPESP's process: 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Antonio Charlys da Costa
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 16/01735-2 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Ester Cerdeira Sabino
• Support type: Regular Research Grants