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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

X-ray microtomography in comparison to radiographic analysis of mechanically damaged maize seeds and its effect on seed germination

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Author(s):
Gomes-Junior, Francisco Guilhien [1] ; Cicero, Silvio Moure [1] ; Pedro Vaz, Carlos Manoel [2] ; Orlandi Lasso, Paulo Renato [2]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Dept Produc Vegetal, Escola Super Agr Luiz de Oueiroz, Av Padua Dias 11, Cx Postal 09, BR-13418900 Piracicaba, SP - Brazil
[2] Embrapa Instrumentacao, Empresa Brasileira Pesquisa Agr, Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Acta Scientiarum. Agronomy; v. 41, 2019.
Web of Science Citations: 0
Abstract

ABSTRACT. Among the most relevant aspects of seed production, mechanical damage may affect seed germination and reduce health and vigor. This study introduces a noninvasive high-resolution imaging procedure for evaluating the mechanical damage to maize seeds and the effects on seed germination. Seeds with different levels of mechanical damage were evaluated using a benchtop micro-computed tomography system (micro-CT) and digital X-ray equipment. The two-dimensional transaxial, coronal and sagittal micro-CT sections were used to inspect the seed anatomy and the mechanical injuries in the internal seed tissue. Germination tests were performed using paper towel rolls (25°C for 7 days) in which the seedling length was evaluated on a daily basis, and the seedling dry biomass was measured at the seventh germination day. The micro-CT cross-sectional images allowed an efficient spatial characterization of the mechanical damage inside the seeds. On average, mechanically damaged seeds produced seedlings with a length 24% shorter and a dry biomass 65% less than that of the undamaged seeds. We concluded that the micro-CT technique provides an efficient means to inspect mechanically damaged maize seeds and allows for a reliable association with germination response. (AU)

FAPESP's process: 12/24683-7 - Comparison between radiological and tomographic methods for evaluation of mechanical damage in seeds
Grantee:Francisco Guilhien Gomes Junior
Support Opportunities: Regular Research Grants
FAPESP's process: 15/24008-6 - Development of imaging analysis techniques to assess the vigor and internal morphology of seeds
Grantee:Francisco Guilhien Gomes Junior
Support Opportunities: Regular Research Grants