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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Probiotic potential and safety of enterococci strains

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Author(s):
Sousa Nascimento, Liane Caroline [1] ; Casarotti, Sabrina Neves [2] ; Todorov, Svetoslav Dimitrov [3] ; Barretto Penna, Ana Lucia [4]
Total Authors: 4
Affiliation:
[1] Fed Inst Maranhao IFMA, BR-65095460 Sao Luis, MA - Brazil
[2] Fed Univ Mato Grosso UFMT, Inst Nat & Exact Sci, BR-78736900 Rondonopolis, MT - Brazil
[3] Univ Sao Paulo, Dept Food Sci & Expt Nutr, BR-05508000 Sao Paulo, SP - Brazil
[4] Sao Paulo State Univ UNESP, Dept Food Engn & Technol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: ANNALS OF MICROBIOLOGY; v. 69, n. 3, p. 241-252, MAR 2019.
Web of Science Citations: 2
Abstract

The aims of this research were to evaluate the safety and probiotic potential of Enterococcus spp. strains and select novel strains for future development of new functional fermented products. Bile salt hydrolase (BSH) activity, capacity of auto-aggregation and co-aggregation, hydrophobicity, tolerance to different pH values and NaCl content, mucin degradation, and antibiotic susceptibility were evaluated. Considering the preliminary probiotic features and safety, the strains were selected for complementary tests: tolerance to gastrointestinal tract (GIT) conditions, adhesion to Caco-2 cells and -galactosidase activity, and presence of genes encoding virulence factors, antibiotic resistance, and biogenic amines were also performed for the selected strains. Enterococcus faecium SJRP20 and SJRP65 resisted well to the GIT conditions, presented low adhesion property, produced -galactosidase although they did not present genes implicated in adhesion, aggregation, and colonization. Enterococcus faecium SJRP65 showed fewer genes related to antibiotic resistance and virulence factors and presented good functional properties, with interesting features for future application in dairy products. (AU)

FAPESP's process: 14/02131-8 - Selection and incorporation of a strain with probiotic potential to a food matrix supplemented with dietary fiber and effect of the symbiotic fermented product on the intestinal microbiota in the simulator of the human microbial ecosystem
Grantee:Ana Lúcia Barretto Penna
Support type: Regular Research Grants