GRIN2B promoter methylation deficits in early-onset schizophrenia and its association with cognitive function

Author(s)

Fachim, Helene A. ^{[1, 2]} ; Loureiro, Camila M. ^{[3, 4]} ; Corsi-Zuelli, Fabiana ^[1] ; Shuhama, Rosana ^{[1, 3]} ; Louzada, Jr., Paulo ^[2] ; Menezes, Paulo Rossi ^{[5, 3]} ; Dalton, Caroline F. ^[2] ; Del-Ben, Cristina Marta ^{[1, 3]} ; Reynolds, Gavin P. ^[2]

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Neurosci & Behav, Sao Paulo - Brazil

^[2] Sheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire - England

^[3] Univ Sao Paulo, Populat Mental Hlth Ctr, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Div Clin Immunol, Dept Internal Med, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Med, Dept Prevent Med, Sao Paulo - Brazil

• Document type: Journal article
• Source: Epigenomics; v. 11, n. 4, p. 401-410, MAR 2019.
• Web of Science Citations: 1

Abstract

Aim: We investigated GRIN1 and GRIN2B promoter methylation in first-episode schizophrenia patients compared with siblings and controls, testing for correlations between DNA methylation, cognitive performance and clinical variables. Materials \& methods: Blood-derived DNA from all groups underwent bisulfite conversion and pyrosequencing to determine methylation at CpG sites within the GRIN1 and GRIN2B promoters and results were compared with the measure of global methylation LINE-7.Results: We found hypomethylation among all CpGs analyzed within GRIN2B promoter in patients and greater LINE-1 methylation in patients and siblings. CpG4 was correlated to a measure of intellectual function. Conclusion: Changes in GRIN2B promoter methylation may represent an environmental influence contributing to glutamatergic dysfunction in psychosis and relate to lower cognitive performance in subjects with first-episode schizophrenia. (AU)

• FAPESP's process: 12/05178-0 - Schizophrenia and other psychotic disorders: social and biological determinants
• Grantee: Paulo Rossi Menezes
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 12/17626-7 - Cellular and molecular mechanisms involved in the role of atypical neurotransmitters in neuropsychiatric disorders
• Grantee: Francisco Silveira Guimaraes
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/12195-9 - Impact of early life stress in the inflammatory profile of first episode of psychosis (FEP) patients and in the animal model of post-weaning social isolation
• Grantee: Fabiana Maria das Graças Corsi Zuelli
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/11167-3 - Gene-environment interactions in Schizophrenia and other psychotic disorders
• Grantee: Rosana Shuhama
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/08216-2 - CRID - Center for Research in Inflammatory Diseases
• Grantee: Fernando de Queiroz Cunha
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 17/17480-6 - Impact of early life stress in the inflammatory profile of first episode of psychosis (FEP) patients and in the animal model of post-weaning social isolation
• Grantee: Fabiana Maria das Graças Corsi Zuelli
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 15/02948-7 - Study of epigenetic alterations in GABAergic neurotransmission in schizophrenia and other psychoses and in animals undergoing social isolation from weaning
• Grantee: Helene Aparecida Fachim
• Support Opportunities: Scholarships in Brazil - Post-Doctoral