Transcriptome sequencing reveals genes and adaptation pathways in Salmonella Typhimurium inoculated in four low water activity foods

Author(s)

Crucello, Aline ^[1] ; Furtado, Marianna M. ^[1] ; Chaves, Monyca D. R. ^[1] ; Sant'Ana, Anderson S. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Fac Food Engn, Dept Food Sci, Campinas, SP - Brazil

• Document type: Journal article
• Source: FOOD MICROBIOLOGY; v. 82, p. 426-435, SEP 2019.
• Web of Science Citations: 3

Abstract

Salmonella enterica serotypes have been reported as the agent of various outbreaks occurred after the consumption of low water activity (a(w)) foods. When the pathogen encounters harsh conditions, several regulatory networks are activated through dynamic differential gene expression that lead to cell survival for prolonged periods. In this work, the transcriptome of S. enterica serovar Typhimurium using RNA-Seq, after cells' inoculation in four distinct types of low a(w) foods ( milk chocolate, powdered milk, black pepper, and dried pet food), following storage at 25 degrees C per 24 and 72 h was studied. The findings of this study suggest that gene regulation is influenced by the food composition mainly in the first 24 h post-inoculum, proceeded by the induction of similar genes shared among all samples. It was possible to evaluate the differences on each type of food matrix regarding the bacteria adaptation, as well as the similarities provoked by low a(w). The results reveal genes that may play key roles in response to desiccation in Salmonella, as well as the pathways in which they are involved. (AU)

• FAPESP's process: 14/17387-8 - Identification and functional analysis of new genes and regulatory elements implicated in the survival and persistence of Salmonella enterica in low-water activity food
• Grantee: Aline Crucello
• Support Opportunities: Scholarships in Brazil - Post-Doctoral