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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Coffee, but Neither Decaffeinated Coffee nor Caffeine, Elicits Chemoprotection Against a Direct Carcinogen in the Colon of Wistar Rats

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Author(s):
Soares, Paulo Victoria [1] ; Kannen, Vinicius [2] ; Jordao Junior, Alceu Afonso [3] ; Garcia, Sergio Britto [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Dept Pathol, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Dept Toxicol Bromatol & Clin Anal, Ribeirao Preto - Brazil
[3] Univ Sao Paulo, Dept Internal Med, Ribeirao Preto - Brazil
Total Affiliations: 3
Document type: Journal article
Source: NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL; v. 71, n. 4, p. 615-623, MAY 19 2019.
Web of Science Citations: 2
Abstract

Colorectal cancer (CRC) is the third most frequent malignancy worldwide. Coffee is the second most consumed drink in the globe and suggested to decrease the CRC risk. Here, we explored whether coffee, decaffeinated coffee, or caffeine impact on the development of colorectal carcinogenesis induced by the direct carcinogen N-methyl-N-nitro-N-nitrosoguanidine (MNNG) in rats. To this end, sixty-four young male Wistar rats were divided into eight groups of eight animals each. We analyzed the frequency of dysplastic crypts and expression of metallothionein as a biomarker of the cancer risk, as well the expression of phosphorylated H2A histone family/member X (gamma H2AX) for DNA damage and cyclooxygenase-2 (COX-2) for inflammatory response. We also studied the oxidative stress profile in hepatic and colonic frozen samples (malondialdehyde {[}MDA], glutathione {[}GSH], and alpha-tocopherol). We found that coffee but neither decaffeinated coffee nor caffeine decreased the development of dysplastic crypts in MNNG-exposed rats. All treatments reduced DNA damage intensity in colonocytes. Only decaffeinated coffee increased the numbers of metallothionein positive crypts in comparison with coffee-treated rats. Coffee and caffeine inhibited COX-2 expression in the colon. Both decaffeinated coffee and caffeine decreased hepatic alpha-tocopherol levels. We suggest that coffee may have other compounds that elicit greater chemoprotective effects than caffeine reducing the CRC risk. (AU)

FAPESP's process: 14/06428-5 - Differentiating the effects of epithelial from the neural serotoninergic signalling during inflammation-related colon cancer
Grantee:Vinicius Kannen Cardoso
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 15/01723-1 - Differentiating the effects of epithelial from the neural serotonergic signalling during the development of colon carcinogenesis
Grantee:Vinicius Kannen Cardoso
Support Opportunities: Scholarships in Brazil - Young Researchers